Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS14855 and AZOBR_RS14860 are separated by 16 nucleotidesAZOBR_RS14860 and AZOBR_RS14865 are separated by 1429 nucleotidesAZOBR_RS14865 and AZOBR_RS14870 are separated by 85 nucleotidesAZOBR_RS14870 and AZOBR_RS14875 are separated by 129 nucleotides AZOBR_RS14855: AZOBR_RS14855 - hypothetical protein, at 105,518 to 105,925 _RS14855 AZOBR_RS14860: AZOBR_RS14860 - hypothetical protein, at 105,942 to 106,298 _RS14860 AZOBR_RS14865: AZOBR_RS14865 - aminopeptidase N, at 107,728 to 110,385 _RS14865 AZOBR_RS14870: AZOBR_RS14870 - cytochrome C transmembrane protein, at 110,471 to 110,851 _RS14870 AZOBR_RS14875: AZOBR_RS14875 - carbon monoxide dehydrogenase subunit G, at 110,981 to 111,643 _RS14875
Group Condition AZOBR_RS14855 AZOBR_RS14860 AZOBR_RS14865 AZOBR_RS14870 AZOBR_RS14875
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.3 N.D. -0.2 -7.5 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.2 N.D. -0.2 -7.1 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.1 N.D. -0.3 -5.7 +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.6 N.D. -0.2 -4.2 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.1 N.D. -0.2 -4.4 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.2 N.D. -0.1 -4.6 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.5 N.D. -0.2 -3.9 +0.0
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.5 N.D. -0.1 -4.0 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.0 N.D. -0.0 -3.9 +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.3 N.D. -0.2 -3.0 +0.1
stress 2-pentyl-furan 1.335528 mM -0.9 N.D. +0.0 -1.6 -0.5
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -1.0 N.D. -0.1 -2.0 +0.6
stress 1-octen-3-ol 0.6784116667 mM -1.2 N.D. -0.2 -1.3 +0.2
stress 2-pentyl-furan 1.335528 mM -1.4 N.D. -0.3 -0.2 -0.6
stress 3-octanone 4.2317 mM -1.0 N.D. -0.2 -0.9 +0.1
stress 3-octanone 6.3476 mM -0.6 N.D. +0.2 -1.6 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 N.D. -0.2 +0.9 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. -0.2 +0.7 -0.6
stress 2-pentyl-furan 1.335528 mM -1.1 N.D. +0.1 +1.0 -0.5
motility inner cut, LB soft agar motility assay +1.4 N.D. -0.0 -1.8 +0.0
lb no stress control LB no stress control -0.8 N.D. -0.1 +0.9 -0.3
stress 2-pentyl-furan 1.780666667 mM -0.8 N.D. +0.1 +1.0 -0.5
stress 2-Furfuraldehyde 0.2 vol% -1.7 N.D. +0.1 +1.4 +0.1
motility inner cut, LB soft agar motility assay +0.9 N.D. +0.1 -0.9 -0.1
stress D-Cycloserine 0.125 mg/ml +1.9 N.D. -0.0 -0.6 -0.1
motility inner cut, LB soft agar motility assay +1.5 N.D. +0.2 -0.2 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +1.5 N.D. -0.2 -0.1 +0.5
stress Sisomicin 0.01 mg/ml +1.5 N.D. -0.2 +0.5 +0.2
lb LB +2.1 N.D. +0.0 +0.7 -0.6
lb LB +1.7 N.D. -0.0 +0.6 +0.5
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