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Fitness for 5 genes in
Azospirillum brasilense Sp245
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
AZOBR_RS14505 and AZOBR_RS14510 are separated by 11 nucleotides
AZOBR_RS14510 and AZOBR_RS14515 are separated by 10 nucleotides
AZOBR_RS14515 and AZOBR_RS14520 overlap by 4 nucleotides
AZOBR_RS14520 and AZOBR_RS14525 are separated by 1 nucleotides
AZOBR_RS14505: AZOBR_RS14505 - hydrogenase, at 23,600 to 24,121
_RS14505
AZOBR_RS14510: AZOBR_RS14510 - hydrogenase expression protein HypE, at 24,133 to 25,674
_RS14510
AZOBR_RS14515: AZOBR_RS14515 - oxidoreductase, at 25,685 to 27,142
_RS14515
AZOBR_RS14520: AZOBR_RS14520 - hydrogenase-4 component E, at 27,139 to 27,801
_RS14520
AZOBR_RS14525: AZOBR_RS14525 - formate hydrogenlyase, at 27,803 to 28,720
_RS14525
Group
Condition
AZOBR
_RS14505
AZOBR
_RS14510
AZOBR
_RS14515
AZOBR
_RS14520
AZOBR
_RS14525
motility
inner cut, LB soft agar motility assay
-1.8
-0.1
-0.1
+0.4
-0.3
nitrogen source
L-Histidine (N)
-0.4
-0.9
-0.2
-0.0
-0.2
stress
2-pentyl-furan 1.780666667 mM
-0.5
-0.2
+0.0
-0.3
-0.4
nitrogen source
L-Threonine (N)
-1.3
+0.1
+0.0
-0.1
+0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.5
+0.1
-0.2
+0.1
-0.7
nitrogen source
casaminos (N)
-0.7
-0.4
+0.1
+0.3
-0.3
stress
3-octanone 6.3476 mM
+1.1
-0.2
-0.1
-0.1
-1.8
stress
Benzethonium chloride 0.003 mM
-1.0
+0.2
+0.1
+0.2
-0.5
stress
D-Cycloserine 0.125 mg/ml
-0.5
+0.1
+0.3
-0.2
-0.4
nitrogen source
casaminos (N)
-0.7
-0.2
+0.0
+0.1
+0.4
stress
2-pentyl-furan 1.335528 mM
+0.6
-0.4
+0.0
-0.2
-0.5
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB
-0.9
+0.1
+0.1
+0.1
+0.3
nitrogen source
L-Isoleucine (N)
+0.4
+0.1
-0.0
+0.3
-0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.5
-0.5
-0.2
+0.2
+0.2
motility
inner cut, LB soft agar motility assay
+0.7
-0.2
-0.1
-0.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.7
-0.3
-0.1
+0.4
-0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.7
-0.4
+0.1
+0.1
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.2
-0.0
-0.2
+0.4
-0.5
motility
inner cut, LB soft agar motility assay
+0.5
+0.8
-0.1
+0.0
-0.0
nitrogen source
L-Asparagine (N)
+0.6
+0.1
+0.2
-0.1
+0.5
anaerobic; rich media
LB; anaerobic
+0.4
+0.2
+0.2
+0.3
+0.3
nitrogen fixation
L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days
+0.5
+0.0
+0.2
+0.3
+0.4
nitrogen fixation
L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days
+0.5
+0.2
+0.2
+0.3
+0.4
nitrogen fixation
L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days
+0.7
+0.2
+0.2
+0.4
+0.3
stress
Sisomicin 0.01 mg/ml
+0.9
+0.5
+0.2
+0.1
+0.1
nitrogen fixation control
L-Malic (C) with ammonium chloride; anaerobic; 3 days
+0.7
+0.2
+0.5
+0.7
+0.4
nitrogen fixation
D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
+1.3
+0.1
+0.5
+0.6
+0.3
nitrogen fixation control
L-Malic (C) with ammonium chloride; anaerobic; 8 days
+0.3
+0.5
+0.6
+0.7
+0.7
nitrogen fixation control
L-Malic (C) with ammonium chloride; anaerobic; 3 days
+0.9
+0.5
+0.5
+0.5
+0.6
nitrogen fixation control
L-Malic (C) with ammonium chloride; anaerobic; 8 days
+1.1
+0.4
+0.6
+0.7
+0.6
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