Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS14505 and AZOBR_RS14510 are separated by 11 nucleotidesAZOBR_RS14510 and AZOBR_RS14515 are separated by 10 nucleotidesAZOBR_RS14515 and AZOBR_RS14520 overlap by 4 nucleotidesAZOBR_RS14520 and AZOBR_RS14525 are separated by 1 nucleotides AZOBR_RS14505: AZOBR_RS14505 - hydrogenase, at 23,600 to 24,121 _RS14505 AZOBR_RS14510: AZOBR_RS14510 - hydrogenase expression protein HypE, at 24,133 to 25,674 _RS14510 AZOBR_RS14515: AZOBR_RS14515 - oxidoreductase, at 25,685 to 27,142 _RS14515 AZOBR_RS14520: AZOBR_RS14520 - hydrogenase-4 component E, at 27,139 to 27,801 _RS14520 AZOBR_RS14525: AZOBR_RS14525 - formate hydrogenlyase, at 27,803 to 28,720 _RS14525
Group Condition AZOBR_RS14505 AZOBR_RS14510 AZOBR_RS14515 AZOBR_RS14520 AZOBR_RS14525
motility inner cut, LB soft agar motility assay -1.8 -0.1 -0.1 +0.4 -0.3
nitrogen source L-Histidine (N) -0.4 -0.9 -0.2 -0.0 -0.2
stress 2-pentyl-furan 1.780666667 mM -0.5 -0.2 +0.0 -0.3 -0.4
nitrogen source L-Threonine (N) -1.3 +0.1 +0.0 -0.1 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 +0.1 -0.2 +0.1 -0.7
nitrogen source casaminos (N) -0.7 -0.4 +0.1 +0.3 -0.3
stress 3-octanone 6.3476 mM +1.1 -0.2 -0.1 -0.1 -1.8
stress Benzethonium chloride 0.003 mM -1.0 +0.2 +0.1 +0.2 -0.5
stress D-Cycloserine 0.125 mg/ml -0.5 +0.1 +0.3 -0.2 -0.4
nitrogen source casaminos (N) -0.7 -0.2 +0.0 +0.1 +0.4
stress 2-pentyl-furan 1.335528 mM +0.6 -0.4 +0.0 -0.2 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.9 +0.1 +0.1 +0.1 +0.3
nitrogen source L-Isoleucine (N) +0.4 +0.1 -0.0 +0.3 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.5 -0.2 +0.2 +0.2
motility inner cut, LB soft agar motility assay +0.7 -0.2 -0.1 -0.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 -0.3 -0.1 +0.4 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.4 +0.1 +0.1 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.2 -0.0 -0.2 +0.4 -0.5
motility inner cut, LB soft agar motility assay +0.5 +0.8 -0.1 +0.0 -0.0
nitrogen source L-Asparagine (N) +0.6 +0.1 +0.2 -0.1 +0.5
anaerobic; rich media LB; anaerobic +0.4 +0.2 +0.2 +0.3 +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.5 +0.0 +0.2 +0.3 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.5 +0.2 +0.2 +0.3 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.7 +0.2 +0.2 +0.4 +0.3
stress Sisomicin 0.01 mg/ml +0.9 +0.5 +0.2 +0.1 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.7 +0.2 +0.5 +0.7 +0.4
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +1.3 +0.1 +0.5 +0.6 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.3 +0.5 +0.6 +0.7 +0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.9 +0.5 +0.5 +0.5 +0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +1.1 +0.4 +0.6 +0.7 +0.6
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