Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS14490 and AZOBR_RS14495 are separated by 72 nucleotidesAZOBR_RS14495 and AZOBR_RS14500 are separated by 176 nucleotidesAZOBR_RS14500 and AZOBR_RS14505 are separated by 7 nucleotidesAZOBR_RS14505 and AZOBR_RS14510 are separated by 11 nucleotides AZOBR_RS14490: AZOBR_RS14490 - hypothetical protein, at 21,806 to 22,114 _RS14490 AZOBR_RS14495: AZOBR_RS14495 - hypothetical protein, at 22,187 to 22,606 _RS14495 AZOBR_RS14500: AZOBR_RS14500 - nucleoside triphosphate pyrophosphohydrolase, at 22,783 to 23,592 _RS14500 AZOBR_RS14505: AZOBR_RS14505 - hydrogenase, at 23,600 to 24,121 _RS14505 AZOBR_RS14510: AZOBR_RS14510 - hydrogenase expression protein HypE, at 24,133 to 25,674 _RS14510
Group Condition AZOBR_RS14490 AZOBR_RS14495 AZOBR_RS14500 AZOBR_RS14505 AZOBR_RS14510
motility inner cut, LB soft agar motility assay N.D. -0.2 -0.1 -1.8 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.7 -0.8 -0.5 +0.1
stress 3-octanone 6.3476 mM N.D. -0.2 -1.2 -0.6 +0.1
nitrogen source L-Threonine (N) N.D. -0.0 -0.4 -1.3 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.9 -0.0 -0.9 +0.1
stress 3-octanone 5.2896 mM N.D. +0.0 -0.3 -0.8 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.5 -0.2 -0.1 -0.5
stress 3-octanone 5.2896 mM N.D. +0.1 -0.5 -0.5 -0.3
stress Benzethonium chloride 0.003 mM N.D. +0.5 -0.6 -1.0 +0.2
nitrogen source L-Histidine (N) N.D. +0.1 +0.2 -0.4 -0.9
stress 2-pentyl-furan 1.335528 mM N.D. -0.4 -0.6 +0.2 -0.1
nitrogen source casaminos (N) N.D. +0.3 +0.1 -0.7 -0.4
stress 3-octanone 4.2317 mM N.D. +0.1 -1.0 +0.4 -0.2
nitrogen source L-Phenylalanine (N) N.D. +0.3 +0.3 -1.0 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.8 +0.4 +0.7 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 +0.5 -0.2 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.5 +0.0 +0.7 +0.2
stress 3-octanone 6.3476 mM N.D. +0.0 -0.4 +1.1 -0.2
stress 2-pentyl-furan 1.335528 mM N.D. +0.2 +0.3 +0.6 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.6 +0.2 +0.5 -0.5
motility inner cut, LB soft agar motility assay N.D. +0.5 -0.1 +0.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.7 +0.2 +0.4 -0.2
motility inner cut, LB soft agar motility assay N.D. -0.4 +0.3 +0.5 +0.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -0.1 +0.1 +0.9 +0.5
stress 2-Furfuraldehyde 0.2 vol% N.D. +0.1 +0.3 +0.3 +0.6
lb LB N.D. -0.0 +0.7 +0.3 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.0 +0.2 +1.2 -0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.0 +0.0 +1.1 +0.4
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. +0.0 +0.2 +1.3 +0.1
stress Sisomicin 0.01 mg/ml N.D. +0.8 -0.2 +0.9 +0.5
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