Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS14445 and AZOBR_RS14450 are separated by 305 nucleotidesAZOBR_RS14450 and AZOBR_RS14455 are separated by 42 nucleotidesAZOBR_RS14455 and AZOBR_RS14460 overlap by 4 nucleotidesAZOBR_RS14460 and AZOBR_RS14465 are separated by 95 nucleotides AZOBR_RS14445: AZOBR_RS14445 - hypothetical protein, at 14,687 to 14,881 _RS14445 AZOBR_RS14450: AZOBR_RS14450 - potassium transporter TrkA, at 15,187 to 16,563 _RS14450 AZOBR_RS14455: AZOBR_RS14455 - D-amino acid aminotransferase, at 16,606 to 17,469 _RS14455 AZOBR_RS14460: AZOBR_RS14460 - HAD family hydrolase, at 17,466 to 18,164 _RS14460 AZOBR_RS14465: AZOBR_RS14465 - RNA-binding protein Hfq, at 18,260 to 18,508 _RS14465
Group Condition AZOBR_RS14445 AZOBR_RS14450 AZOBR_RS14455 AZOBR_RS14460 AZOBR_RS14465
motility outer cut, LB soft agar motility assay N.D. -2.5 N.D. -1.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.6 N.D. -2.5 N.D.
nitrogen source L-Phenylalanine (N) N.D. -1.4 N.D. -1.2 N.D.
nitrogen source L-Glutamic (N) N.D. -2.5 N.D. +0.0 N.D.
nitrogen source L-Isoleucine (N) N.D. -3.2 N.D. +1.0 N.D.
stress Sisomicin 0.01 mg/ml N.D. -0.5 N.D. -1.4 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. -1.8 N.D. -0.1 N.D.
stress 3-octanone 6.3476 mM N.D. -1.2 N.D. -0.4 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.4 N.D. -2.0 N.D.
stress 3-octanone 6.3476 mM N.D. -1.0 N.D. -0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.7 N.D. -0.9 N.D.
carbon source pyruvate (C) N.D. -1.2 N.D. -0.4 N.D.
carbon source a-Ketoglutaric (C) N.D. -1.2 N.D. -0.4 N.D.
carbon source L-Proline (C) N.D. -0.6 N.D. -0.9 N.D.
carbon source D-Fructose (C) N.D. -1.5 N.D. +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.7 N.D. -2.0 N.D.
stress Benzethonium chloride 0.003 mM N.D. +0.5 N.D. -1.5 N.D.
carbon source L-Malic (C) N.D. +0.6 N.D. -1.4 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. +1.0 N.D. -1.0 N.D.
stress D-Cycloserine 0.125 mg/ml N.D. +0.9 N.D. -0.6 N.D.
stress 2-Furfuraldehyde 0.2 vol% N.D. +1.0 N.D. -0.7 N.D.
stress Cobalt chloride 0.3 mM N.D. +1.6 N.D. -0.4 N.D.
motility inner cut, LB soft agar motility assay N.D. +0.5 N.D. +1.0 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.4 N.D. +1.5 N.D.
stress Paraquat dichloride 0.02 mg/ml N.D. +1.1 N.D. +0.9 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.2 N.D. +1.8 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.1 N.D. +1.9 N.D.
lb LB N.D. +1.4 N.D. +0.8 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.4 N.D. +1.9 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.5 N.D. +2.4 N.D.
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