Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS13510 and AZOBR_RS13515 are separated by 130 nucleotidesAZOBR_RS13515 and AZOBR_RS13520 are separated by 8 nucleotidesAZOBR_RS13520 and AZOBR_RS13525 overlap by 4 nucleotidesAZOBR_RS13525 and AZOBR_RS13530 are separated by 305 nucleotides AZOBR_RS13510: AZOBR_RS13510 - threonyl-tRNA synthetase, at 2,866,806 to 2,868,770 _RS13510 AZOBR_RS13515: AZOBR_RS13515 - diguanylate phosphodiesterase, at 2,868,901 to 2,870,136 _RS13515 AZOBR_RS13520: AZOBR_RS13520 - dihydroorotate dehydrogenase, at 2,870,145 to 2,871,218 _RS13520 AZOBR_RS13525: AZOBR_RS13525 - glutathione S-transferase, at 2,871,215 to 2,871,577 _RS13525 AZOBR_RS13530: AZOBR_RS13530 - hypothetical protein, at 2,871,883 to 2,872,131 _RS13530
Group Condition AZOBR_RS13510 AZOBR_RS13515 AZOBR_RS13520 AZOBR_RS13525 AZOBR_RS13530
stress 2-pentyl-furan 1.335528 mM N.D. +0.0 -1.8 -4.7 -0.0
stress 3-octanone 6.3476 mM N.D. -4.2 -1.9 +0.2 -0.1
nitrogen source D-Alanine (N) N.D. -0.1 -4.1 -1.2 -0.1
carbon source a-Ketoglutaric (C) N.D. -0.8 -3.7 -0.7 -0.4
carbon source a-Ketoglutaric (C) N.D. -0.3 -4.2 -0.6 -0.3
carbon source pyruvate (C) N.D. -0.1 -3.0 -1.8 +0.0
carbon source D-Lactate (C) N.D. +0.1 -3.9 -1.0 -0.1
nitrogen source L-Asparagine (N) N.D. -0.5 -2.9 -1.2 -0.1
nitrogen source L-Lysine (N) N.D. +0.7 -3.6 -1.6 -0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -0.5 -4.6 +1.1 -0.6
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. -0.5 -3.3 +0.0 -0.8
nitrogen source L-Threonine (N) N.D. +0.8 -3.8 -1.4 -0.1
nitrogen source L-Lysine (N) N.D. +0.2 -3.4 -1.1 -0.1
carbon source succinate (C) N.D. +0.1 -4.1 -0.2 -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -1.3 -3.4 +1.1 -0.6
carbon source L-Lactate (C) N.D. +1.0 -3.9 -1.0 -0.1
carbon source L-Glutamine (C) N.D. +0.7 -3.7 -0.9 -0.2
stress 2-pentyl-furan 2.671 mM N.D. +0.2 -0.1 -3.3 -0.8
carbon source L-Malic (C) N.D. +0.6 -3.8 -0.9 +0.0
carbon source D,L-Lactate (C) N.D. +0.7 -3.4 -1.1 -0.2
nitrogen source L-Citrulline (N) N.D. +0.5 -3.4 -0.8 -0.1
nitrogen source L-Isoleucine (N) N.D. +1.1 -2.0 -2.1 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.6 -1.7 +0.5 +0.2
nitrogen source nitrate (N) N.D. +1.1 -4.0 -0.4 -0.3
nitrogen source Ammonium chloride (N) N.D. +0.8 -3.1 -1.0 -0.2
nitrogen source Urea (N) N.D. +0.9 -3.1 -0.8 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -2.5 -2.0 +1.2 +0.1
nitrogen source L-Glutamic (N) N.D. +1.1 -0.2 -2.7 -0.6
nitrogen source Parabanic Acid (N) N.D. +0.6 -3.2 +0.4 -0.3
nitrogen source L-Phenylalanine (N) N.D. +1.1 -2.1 -1.1 -0.2
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