Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS13070 and AZOBR_RS13075 are separated by 28 nucleotidesAZOBR_RS13075 and AZOBR_RS13080 are separated by 309 nucleotidesAZOBR_RS13080 and AZOBR_RS13085 overlap by 4 nucleotidesAZOBR_RS13085 and AZOBR_RS13095 are separated by 1605 nucleotides AZOBR_RS13070: AZOBR_RS13070 - taurine ABC transporter permease, at 2,759,069 to 2,759,815 _RS13070 AZOBR_RS13075: AZOBR_RS13075 - sulfonate ABC transporter ATP-binding protein, at 2,759,844 to 2,760,596 _RS13075 AZOBR_RS13080: AZOBR_RS13080 - putative MFS superfamily multidrug transport protein, at 2,760,906 to 2,762,150 _RS13080 AZOBR_RS13085: AZOBR_RS13085 - putative sulfate ester transport system substrate-binding protein, at 2,762,147 to 2,763,202 _RS13085 AZOBR_RS13095: AZOBR_RS13095 - 4Fe-4S ferredoxin, at 2,764,808 to 2,765,119 _RS13095
Group Condition AZOBR_RS13070 AZOBR_RS13075 AZOBR_RS13080 AZOBR_RS13085 AZOBR_RS13095
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.3 -0.4 +0.1 +0.3 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.0 -0.5 -0.3 +0.4 -0.3
stress 2-Furfuraldehyde 0.2 vol% -0.9 -0.2 +0.1 +0.2 -0.4
stress 2-pentyl-furan 2.671 mM +0.7 -1.0 -0.3 -0.0 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.5 -0.2 -0.1 -0.2
motility inner cut, LB soft agar motility assay -0.7 -0.1 +0.5 -0.2 +0.2
carbon source D-Gluconic Acid (C) +0.4 -0.7 +0.3 -0.2 -0.1
carbon source L-Leucine (C) +0.6 -0.7 -0.1 +0.1 +0.1
motility inner cut, LB soft agar motility assay -0.3 -0.5 +0.5 -0.3 +0.6
stress Sisomicin 0.01 mg/ml -0.4 -0.5 -0.0 +0.4 +0.6
lb LB +0.9 -0.1 -0.3 -0.1 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 +0.6 -0.4 +0.3 +0.1
nitrogen source L-Glutamic (N) +0.2 -0.6 -0.3 +0.5 +0.5
motility inner cut, LB soft agar motility assay -0.2 +0.5 -0.4 +0.1 +0.4
lb no stress control LB no stress control -0.3 +0.5 +0.4 -0.2 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.1 +1.3 +0.1 -0.4 -0.3
carbon source Ethanol (C) +0.7 -0.4 -0.1 +0.3 +0.3
stress Benzethonium chloride 0.003 mM -0.1 +0.8 +0.5 -0.2 -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.5 +1.0 -0.0 -0.1 -0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.1 +0.8 +0.3 +0.2 +0.3
stress D-Cycloserine 0.125 mg/ml -0.2 +0.5 +0.7 +0.5 +0.2
anaerobic; rich media LB; anaerobic +0.4 +0.5 +0.3 +0.5 +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.3 +0.6 +0.3 +0.5 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.2 +1.1 +0.2 +0.2 +0.4
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.0 +1.3 +0.3 +0.4 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.4 +0.9 +0.5 +0.2 +0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.7 +1.3 +0.1 +0.3 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.6 +0.9 +0.4 +0.4 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.3 +1.2 +0.6 +0.6 +0.9
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.8 +1.2 +0.8 +0.5 +0.4
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