Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS12010 and AZOBR_RS12015 are separated by 220 nucleotidesAZOBR_RS12015 and AZOBR_RS12020 are separated by 20 nucleotidesAZOBR_RS12020 and AZOBR_RS12025 are separated by 23 nucleotidesAZOBR_RS12025 and AZOBR_RS12030 are separated by 126 nucleotides AZOBR_RS12010: AZOBR_RS12010 - ribonuclease T, at 2,524,312 to 2,524,965 _RS12010 AZOBR_RS12015: AZOBR_RS12015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 2,525,186 to 2,526,529 _RS12015 AZOBR_RS12020: AZOBR_RS12020 - acetyl-CoA carboxylase, at 2,526,550 to 2,526,999 _RS12020 AZOBR_RS12025: AZOBR_RS12025 - 3-dehydroquinate dehydratase, at 2,527,023 to 2,527,472 _RS12025 AZOBR_RS12030: AZOBR_RS12030 - amino acid ABC transporter substrate-binding protein, at 2,527,599 to 2,528,378 _RS12030
Group Condition AZOBR_RS12010 AZOBR_RS12015 AZOBR_RS12020 AZOBR_RS12025 AZOBR_RS12030
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 N.D. N.D. N.D. -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 N.D. N.D. N.D. -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.9 N.D. N.D. N.D. -0.2
motility inner cut, LB soft agar motility assay -0.8 N.D. N.D. N.D. -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.7 N.D. N.D. N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. N.D. N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. N.D. N.D. -0.2
stress Paraquat dichloride 0.02 mg/ml +0.2 N.D. N.D. N.D. -0.5
stress 2-pentyl-furan 1.780666667 mM -0.6 N.D. N.D. N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. N.D. N.D. -0.5
stress 3-octanone 6.3476 mM +0.4 N.D. N.D. N.D. -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 N.D. N.D. N.D. -0.4
nitrogen source L-Histidine (N) +0.3 N.D. N.D. N.D. -0.3
lb LB +0.7 N.D. N.D. N.D. -0.6
stress 2-pentyl-furan 2.671 mM +0.7 N.D. N.D. N.D. -0.6
nitrogen source L-Isoleucine (N) -0.2 N.D. N.D. N.D. +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 N.D. N.D. N.D. -0.2
nitrogen source L-Leucine (N) +0.8 N.D. N.D. N.D. -0.4
carbon source L-Lactate (C) +0.6 N.D. N.D. N.D. +0.1
motility inner cut, LB soft agar motility assay +0.7 N.D. N.D. N.D. -0.1
stress Chlorite 1 mM +0.6 N.D. N.D. N.D. +0.0
nitrogen source L-Phenylalanine (N) +0.4 N.D. N.D. N.D. +0.3
carbon source a-Ketoglutaric (C) +0.2 N.D. N.D. N.D. +0.5
stress Benzethonium chloride 0.003 mM +0.5 N.D. N.D. N.D. +0.3
nitrogen source Cytosine (N) +0.7 N.D. N.D. N.D. +0.1
carbon source a-Ketoglutaric (C) +0.3 N.D. N.D. N.D. +0.5
carbon source D-Gluconic Acid (C) +0.7 N.D. N.D. N.D. +0.1
carbon source Glycerol (C) +0.8 N.D. N.D. N.D. +0.1
motility inner cut, LB soft agar motility assay +0.7 N.D. N.D. N.D. +0.3
motility outer cut, LB soft agar motility assay +0.5 N.D. N.D. N.D. +0.4
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