Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntGFF2272 and GFF2273 are separated by 100 nucleotidesGFF2273 and AZOBR_RS11880 are separated by 19 nucleotidesAZOBR_RS11880 and AZOBR_RS11885 are separated by 169 nucleotidesAZOBR_RS11885 and AZOBR_RS11890 are separated by 81 nucleotides GFF2272 - gamma-glutamyltranspeptidase, at 2,497,219 to 2,498,248 GFF2272 GFF2273 - gamma-glutamyltranspeptidase, at 2,498,349 to 2,498,598 GFF2273 AZOBR_RS11880: AZOBR_RS11880 - 1-aminocyclopropane-1-carboxylate deaminase, at 2,498,618 to 2,499,772 _RS11880 AZOBR_RS11885: AZOBR_RS11885 - glutamate--cysteine ligase, at 2,499,942 to 2,501,306 _RS11885 AZOBR_RS11890: AZOBR_RS11890 - 16S rRNA methyltransferase, at 2,501,388 to 2,502,182 _RS11890
Group Condition GFF2272 GFF2273 AZOBR_RS11880 AZOBR_RS11885 AZOBR_RS11890
stress 2-pentyl-furan 1.335528 mM -2.4 N.D. +0.0 N.D. +0.6
nitrogen source L-Isoleucine (N) -1.8 N.D. -0.1 N.D. +0.1
carbon source D-Gluconic Acid (C) -0.4 N.D. -0.4 N.D. -0.5
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM -1.1 N.D. -0.2 N.D. +0.2
stress 2-pentyl-furan 2.671 mM -1.3 N.D. -0.1 N.D. +0.4
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -1.0 N.D. +0.0 N.D. +0.3
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -1.0 N.D. +0.2 N.D. +0.2
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -0.8 N.D. +0.1 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.2 N.D. +0.2 N.D. +0.8
stress 3-octanone 6.3476 mM -0.8 N.D. +0.0 N.D. +0.6
stress Paraquat dichloride 0.04 mg/ml -0.5 N.D. -0.2 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. -0.7 N.D. +0.4
stress Sisomicin 0.01 mg/ml -0.5 N.D. +0.2 N.D. +0.6
stress 3-octanone 6.3476 mM -0.7 N.D. +0.1 N.D. +0.9
motility inner cut, LB soft agar motility assay -0.8 N.D. +0.0 N.D. +1.1
stress Cobalt chloride 0.2 mM -0.6 N.D. -0.1 N.D. +1.1
stress 3-octanone 6.3476 mM -0.6 N.D. +0.1 N.D. +1.0
stress Bacitracin 0.25 mg/ml +0.9 N.D. -0.1 N.D. -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.5 N.D. -0.5 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 N.D. -0.3 N.D. +0.5
lb LB -0.1 N.D. -0.1 N.D. +1.0
motility inner cut, LB soft agar motility assay -0.3 N.D. +0.1 N.D. +1.0
lb LB -0.4 N.D. +0.5 N.D. +0.9
carbon source a-Ketoglutaric (C) +0.6 N.D. -0.1 N.D. +0.6
motility outer cut, LB soft agar motility assay +0.4 N.D. +0.3 N.D. +0.6
carbon source Glycerol (C) +0.7 N.D. +0.2 N.D. +0.3
nitrogen source L-Glutamic (N) +0.8 N.D. +0.4 N.D. +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +1.3 N.D. +0.1 N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.6 N.D. -0.3 N.D. +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.9 N.D. +0.1 N.D. +0.9
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