Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10720 and AZOBR_RS10725 are separated by 171 nucleotidesAZOBR_RS10725 and AZOBR_RS10730 are separated by 96 nucleotidesAZOBR_RS10730 and AZOBR_RS10735 are separated by 41 nucleotidesAZOBR_RS10735 and AZOBR_RS10740 are separated by 9 nucleotides AZOBR_RS10720: AZOBR_RS10720 - enoyl-ACP reductase, at 2,252,421 to 2,253,269 _RS10720 AZOBR_RS10725: AZOBR_RS10725 - hypothetical protein, at 2,253,441 to 2,254,604 _RS10725 AZOBR_RS10730: AZOBR_RS10730 - chorismate synthase, at 2,254,701 to 2,255,777 _RS10730 AZOBR_RS10735: AZOBR_RS10735 - hypothetical protein, at 2,255,819 to 2,256,352 _RS10735 AZOBR_RS10740: AZOBR_RS10740 - regulator, at 2,256,362 to 2,257,456 _RS10740
Group Condition AZOBR_RS10720 AZOBR_RS10725 AZOBR_RS10730 AZOBR_RS10735 AZOBR_RS10740
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 N.D. -2.2 -0.5
stress 2-pentyl-furan 1.780666667 mM N.D. +0.1 N.D. -2.4 +0.2
motility inner cut, LB soft agar motility assay N.D. +0.1 N.D. -2.2 +0.0
lb LB N.D. +0.1 N.D. -0.5 -0.8
stress 3-octanone 5.2896 mM N.D. -0.4 N.D. -1.1 +0.3
stress 2-pentyl-furan 1.780666667 mM N.D. -0.2 N.D. -0.8 -0.1
nitrogen source L-Asparagine (N) N.D. -0.1 N.D. -1.1 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.2 N.D. -1.0 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.1 N.D. -1.0 +0.3
nitrogen source L-Serine (N) N.D. -0.3 N.D. -0.9 +0.4
stress 2-pentyl-furan 1.780666667 mM N.D. -0.2 N.D. -1.2 +0.6
stress 3-octanone 5.2896 mM N.D. -0.3 N.D. -0.7 +0.3
stress 2-pentyl-furan 2.671 mM N.D. -0.6 N.D. -0.8 +0.9
stress 1-octen-3-ol 0.6784116667 mM N.D. -0.7 N.D. +0.3 +0.2
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM N.D. -0.2 N.D. -0.5 +0.5
stress Benzethonium chloride 0.003 mM N.D. -0.6 N.D. +0.2 +0.5
stress 2-pentyl-furan 1.335528 mM N.D. +0.4 N.D. -0.8 +0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.2 N.D. +0.5 -0.5
motility inner cut, LB soft agar motility assay N.D. -0.3 N.D. +0.8 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. -0.1 N.D. +0.8 -0.3
stress Paraquat dichloride 0.04 mg/ml N.D. +0.2 N.D. +0.8 -0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.2 N.D. +0.8 -0.3
nitrogen source Parabanic Acid (N) N.D. -0.3 N.D. +1.0 +0.3
nitrogen source Cytosine (N) N.D. -0.0 N.D. +0.7 +0.4
anaerobic; rich media LB; anaerobic N.D. -0.1 N.D. +1.2 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.4 N.D. +0.8 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.3 N.D. +0.9 +0.1
nitrogen source L-Glutamic (N) N.D. +0.2 N.D. +0.7 +0.5
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. +0.1 N.D. +0.9 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.8 N.D. +0.7 +0.2
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