Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10695 and AZOBR_RS10705 are separated by 1296 nucleotidesAZOBR_RS10705 and AZOBR_RS10710 are separated by 137 nucleotidesAZOBR_RS10710 and AZOBR_RS10715 are separated by 19 nucleotidesAZOBR_RS10715 and AZOBR_RS10720 are separated by 136 nucleotides AZOBR_RS10695: AZOBR_RS10695 - potassium transporter TrkH, at 2,245,961 to 2,247,331 _RS10695 AZOBR_RS10705: AZOBR_RS10705 - hypothetical protein, at 2,248,628 to 2,249,764 _RS10705 AZOBR_RS10710: AZOBR_RS10710 - radical SAM domain-containing protein, at 2,249,902 to 2,250,918 _RS10710 AZOBR_RS10715: AZOBR_RS10715 - membrane protein, at 2,250,938 to 2,252,284 _RS10715 AZOBR_RS10720: AZOBR_RS10720 - enoyl-ACP reductase, at 2,252,421 to 2,253,269 _RS10720
Group Condition AZOBR_RS10695 AZOBR_RS10705 AZOBR_RS10710 AZOBR_RS10715 AZOBR_RS10720
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.4 -1.1 +0.6 -1.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.5 -0.2 -0.0 -1.3 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 -0.4 -0.3 -1.1 N.D.
lb LB +0.6 -1.5 -0.3 -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.7 -0.4 +0.2 -0.9 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 -0.4 -0.1 -0.9 N.D.
stress D-Cycloserine 0.125 mg/ml -0.6 -0.5 +0.1 -0.7 N.D.
carbon source D-Gluconic Acid (C) -0.6 -0.6 -0.1 -0.2 N.D.
motility inner cut, LB soft agar motility assay +0.2 -1.0 -0.2 -0.5 N.D.
nitrogen source D-Alanine (N) -0.3 -0.5 +0.2 -0.6 N.D.
motility outer cut, LB soft agar motility assay -0.2 +0.2 -0.5 -0.7 N.D.
stress Benzethonium chloride 0.003 mM -0.0 -0.5 +0.2 -0.8 N.D.
nitrogen source L-Glutamic (N) -0.6 -0.6 +0.0 +0.1 N.D.
motility inner cut, LB soft agar motility assay +0.2 -1.0 -0.1 -0.1 N.D.
carbon source D-Gluconic Acid (C) -0.1 -0.8 -0.2 +0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.4 -0.8 +0.1 -0.5 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.2 -0.7 +0.1 -0.6 N.D.
nitrogen source L-Isoleucine (N) -0.0 -0.8 +0.3 -0.2 N.D.
stress 2-pentyl-furan 1.335528 mM +0.1 -1.2 +0.0 +0.3 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.2 -0.9 +0.1 -0.2 N.D.
nitrogen source L-Aspartic Acid (N) -0.3 -0.6 +0.2 -0.1 N.D.
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.5 -1.2 +0.3 -0.3 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.3 -0.7 -0.3 +0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.5 -0.7 +0.0 -0.3 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.5 -0.7 +0.0 -0.0 N.D.
lb LB +0.8 -0.3 +0.1 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.5 +0.4 +0.1 N.D.
stress Cobalt chloride 0.2 mM +1.0 -0.1 -0.2 -0.1 N.D.
stress Cobalt chloride 0.3 mM +1.0 +0.4 +0.0 -0.0 N.D.
stress Paraquat dichloride 0.04 mg/ml +0.6 +0.6 +0.5 -0.1 N.D.
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