Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10625 and AZOBR_RS10630 are separated by 846 nucleotidesAZOBR_RS10630 and AZOBR_RS10635 are separated by 85 nucleotidesAZOBR_RS10635 and AZOBR_RS10640 are separated by 92 nucleotidesAZOBR_RS10640 and AZOBR_RS10645 are separated by 38 nucleotides AZOBR_RS10625: AZOBR_RS10625 - RNA polymerase sigma-70 factor, at 2,231,261 to 2,231,749 _RS10625 AZOBR_RS10630: AZOBR_RS10630 - lytic transglycosylase, at 2,232,596 to 2,233,591 _RS10630 AZOBR_RS10635: AZOBR_RS10635 - hypothetical protein, at 2,233,677 to 2,234,435 _RS10635 AZOBR_RS10640: AZOBR_RS10640 - tRNA-Glu, at 2,234,528 to 2,234,603 _RS10640 AZOBR_RS10645: AZOBR_RS10645 - tRNA-Glu, at 2,234,642 to 2,234,717 _RS10645
Group Condition AZOBR_RS10625 AZOBR_RS10630 AZOBR_RS10635 AZOBR_RS10640 AZOBR_RS10645
stress Sisomicin 0.01 mg/ml -1.9 -0.2 -0.4 -4.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.2 +0.3 -0.1 -1.7 N.D.
stress D-Cycloserine 0.125 mg/ml +0.4 +0.0 +0.0 -4.3 N.D.
motility inner cut, LB soft agar motility assay -4.7 +0.1 +0.0 +0.6 N.D.
stress 2-Furfuraldehyde 0.2 vol% -0.4 -0.2 -0.4 -2.8 N.D.
motility outer cut, LB soft agar motility assay -3.0 -0.3 -0.2 -0.2 N.D.
stress Cobalt chloride 0.2 mM -0.9 -0.2 -0.1 -2.3 N.D.
nitrogen source L-Isoleucine (N) -3.9 +0.1 -0.0 +0.4 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.6 +0.0 -0.0 -2.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.4 -0.2 +0.3 -3.4 N.D.
nitrogen source Cytosine (N) -1.7 +0.2 -0.2 -1.1 N.D.
carbon source L-Malic (C) -2.0 +0.1 -0.1 -0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.8 +0.1 +0.3 -0.2 N.D.
stress Cobalt chloride 0.3 mM -1.7 -0.2 -0.1 -0.5 N.D.
nitrogen source L-Serine (N) -2.1 +0.1 +0.1 -0.4 N.D.
nitrogen source L-Threonine (N) -1.8 -0.0 +0.5 -0.9 N.D.
nitrogen source L-Histidine (N) -0.3 -0.1 -0.3 -1.5 N.D.
lb LB -0.4 -0.1 +0.0 -1.8 N.D.
motility inner cut, LB soft agar motility assay -0.7 -0.3 -0.0 -1.2 N.D.
carbon source Ethanol (C) -0.6 -0.1 +0.1 -1.6 N.D.
stress Vancomycin 0.03 mg/ml -1.1 -0.0 -0.5 -0.5 N.D.
stress Chlorite 1 mM -1.8 -0.1 -0.2 -0.0 N.D.
nitrogen source casaminos (N) -1.7 -0.2 +0.3 -0.5 N.D.
lb LB -2.4 +0.0 +0.1 +0.1 N.D.
nitrogen source Parabanic Acid (N) -1.3 +0.1 +0.0 -0.8 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 +0.0 +0.2 -1.6 N.D.
lb LB -0.9 -0.2 +0.3 -0.9 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.9 +0.5 +0.4 +0.4 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +1.2 -0.2 +0.4 -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +1.7 -0.3 +0.2 -0.1 N.D.
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