Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10610 and AZOBR_RS10615 are separated by 18 nucleotidesAZOBR_RS10615 and AZOBR_RS10620 are separated by 330 nucleotidesAZOBR_RS10620 and AZOBR_RS10625 are separated by 231 nucleotidesAZOBR_RS10625 and AZOBR_RS10630 are separated by 846 nucleotides AZOBR_RS10610: AZOBR_RS10610 - hypothetical protein, at 2,227,787 to 2,229,862 _RS10610 AZOBR_RS10615: AZOBR_RS10615 - exported protein of unknown function, at 2,229,881 to 2,230,534 _RS10615 AZOBR_RS10620: AZOBR_RS10620 - membrane protein, at 2,230,865 to 2,231,029 _RS10620 AZOBR_RS10625: AZOBR_RS10625 - RNA polymerase sigma-70 factor, at 2,231,261 to 2,231,749 _RS10625 AZOBR_RS10630: AZOBR_RS10630 - lytic transglycosylase, at 2,232,596 to 2,233,591 _RS10630
Group Condition AZOBR_RS10610 AZOBR_RS10615 AZOBR_RS10620 AZOBR_RS10625 AZOBR_RS10630
motility inner cut, LB soft agar motility assay -0.1 -0.2 N.D. -4.7 +0.1
nitrogen source L-Isoleucine (N) +0.0 -0.0 N.D. -3.9 +0.1
motility outer cut, LB soft agar motility assay -0.0 -0.2 N.D. -3.0 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.0 N.D. -3.2 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.2 N.D. -2.8 +0.1
lb LB -0.1 -0.0 N.D. -2.4 +0.0
stress Chlorite 1 mM -0.2 -0.1 N.D. -1.8 -0.1
nitrogen source L-Serine (N) +0.1 +0.0 N.D. -2.1 +0.1
stress Sisomicin 0.01 mg/ml +0.2 +0.0 N.D. -1.9 -0.2
carbon source L-Malic (C) -0.0 +0.1 N.D. -2.0 +0.1
stress Cobalt chloride 0.3 mM -0.0 +0.1 N.D. -1.7 -0.2
nitrogen source L-Threonine (N) -0.0 +0.1 N.D. -1.8 -0.0
nitrogen source Cytosine (N) -0.2 -0.1 N.D. -1.7 +0.2
nitrogen source casaminos (N) +0.1 +0.1 N.D. -1.7 -0.2
stress 2-pentyl-furan 1.335528 mM -0.3 -0.1 N.D. -1.2 +0.1
nitrogen source L-Lysine (N) -0.1 -0.0 N.D. -1.3 -0.1
lb LB +0.2 -0.1 N.D. -1.6 +0.1
stress Vancomycin 0.03 mg/ml +0.1 -0.2 N.D. -1.1 -0.0
nitrogen source Parabanic Acid (N) -0.1 +0.1 N.D. -1.3 +0.1
stress Paraquat dichloride 0.04 mg/ml +0.0 -0.3 N.D. -0.9 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -0.1 N.D. -1.1 +0.3
carbon source a-Ketoglutaric (C) -0.0 +0.2 N.D. -1.1 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.4 N.D. -0.8 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.2 +0.2 N.D. -1.1 +0.2
stress 3-octanone 6.3476 mM +0.2 +0.3 N.D. -0.6 -0.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.2 +0.5 N.D. -0.9 +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.4 +0.4 N.D. -0.4 +0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.1 -0.1 N.D. +1.2 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.1 -0.2 N.D. +1.7 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.4 N.D. +1.2 +0.2
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