Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10540 and AZOBR_RS10545 overlap by 4 nucleotidesAZOBR_RS10545 and AZOBR_RS10550 are separated by 399 nucleotidesAZOBR_RS10550 and AZOBR_RS10560 are separated by 1947 nucleotidesAZOBR_RS10560 and AZOBR_RS10565 are separated by 2 nucleotides AZOBR_RS10540: AZOBR_RS10540 - peptidase M16, at 2,210,981 to 2,212,237 _RS10540 AZOBR_RS10545: AZOBR_RS10545 - threonine synthase, at 2,212,234 to 2,213,646 _RS10545 AZOBR_RS10550: AZOBR_RS10550 - 1 4-beta-glucanase, at 2,214,046 to 2,215,137 _RS10550 AZOBR_RS10560: AZOBR_RS10560 - hypothetical protein, at 2,217,085 to 2,217,825 _RS10560 AZOBR_RS10565: AZOBR_RS10565 - hypothetical protein, at 2,217,828 to 2,218,202 _RS10565
Group Condition AZOBR_RS10540 AZOBR_RS10545 AZOBR_RS10550 AZOBR_RS10560 AZOBR_RS10565
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. +0.3 -2.9 -0.2
stress Cobalt chloride 0.2 mM -0.3 N.D. +0.1 -1.2 -0.3
stress Thallium(I) acetate 0.008 mg/ml -0.2 N.D. -0.1 -1.0 -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.2 N.D. +0.0 -1.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. +0.2 -0.8 -0.1
stress Paraquat dichloride 0.02 mg/ml -0.2 N.D. -0.0 +0.4 -0.9
anaerobic; rich media LB; anaerobic +0.6 N.D. +0.2 -0.8 -0.4
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.4 N.D. +0.3 -1.0 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.6 N.D. -0.0 -1.3 +0.5
nitrogen source L-Threonine (N) -0.1 N.D. -0.1 +0.7 -0.6
lb LB +0.2 N.D. +0.2 -0.9 +0.5
nitrogen source Parabanic Acid (N) -0.4 N.D. -0.2 +1.0 -0.2
stress Paraquat dichloride 0.04 mg/ml +0.5 N.D. +0.3 -0.4 +0.2
nitrogen source L-Leucine (N) +0.0 N.D. -0.0 +1.1 -0.4
stress 2-pentyl-furan 2.671 mM +0.2 N.D. -0.1 +0.9 -0.2
carbon source L-Lactate (C) +0.2 N.D. -0.2 +1.0 -0.1
stress 3-octanone 6.3476 mM -0.1 N.D. -0.1 +1.3 -0.4
stress 2-pentyl-furan 1.335528 mM +0.3 N.D. -0.1 +1.2 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 N.D. -0.1 +0.5 +0.5
motility inner cut, LB soft agar motility assay -0.0 N.D. +0.0 +1.3 -0.1
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -0.1 N.D. -0.2 +0.9 +0.6
stress Sisomicin 0.01 mg/ml -0.1 N.D. +0.1 +1.3 +0.0
nitrogen source Parabanic Acid (N) +0.0 N.D. +0.1 +1.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. +0.1 +1.1 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.3 N.D. -0.1 +0.8 +0.4
nitrogen source L-Isoleucine (N) -0.1 N.D. +0.3 +1.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. -0.0 +1.3 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.2 N.D. +0.1 +0.7 +0.7
motility inner cut, LB soft agar motility assay +0.2 N.D. +0.3 +1.0 +0.4
stress 2-pentyl-furan 1.780666667 mM -0.2 N.D. +0.4 +0.7 +1.0
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