Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10520 and AZOBR_RS10525 are separated by 158 nucleotidesAZOBR_RS10525 and AZOBR_RS10530 are separated by 73 nucleotidesAZOBR_RS10530 and AZOBR_RS10535 are separated by 92 nucleotidesAZOBR_RS10535 and AZOBR_RS10540 are separated by 27 nucleotides AZOBR_RS10520: AZOBR_RS10520 - copper-resistance protein, at 2,207,133 to 2,209,217 _RS10520 AZOBR_RS10525: AZOBR_RS10525 - hypothetical protein, at 2,209,376 to 2,209,951 _RS10525 AZOBR_RS10530: AZOBR_RS10530 - hypothetical protein, at 2,210,025 to 2,210,270 _RS10530 AZOBR_RS10535: AZOBR_RS10535 - GCN5 family N-acetyltransferase, at 2,210,363 to 2,210,953 _RS10535 AZOBR_RS10540: AZOBR_RS10540 - peptidase M16, at 2,210,981 to 2,212,237 _RS10540
Group Condition AZOBR_RS10520 AZOBR_RS10525 AZOBR_RS10530 AZOBR_RS10535 AZOBR_RS10540
stress D-Cycloserine 0.125 mg/ml N.D. -0.0 -0.7 +0.1 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 -0.7 -0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.0 -0.9 -0.1 +0.1
nitrogen source L-Lysine (N) N.D. +0.2 +0.1 -0.6 -0.4
motility inner cut, LB soft agar motility assay N.D. +0.3 -0.4 -0.5 -0.0
stress 3-octanone 6.3476 mM N.D. +0.2 -0.4 -0.4 +0.1
stress 2-pentyl-furan 1.335528 mM N.D. +0.0 -0.2 -0.6 +0.3
stress 3-octanone 6.3476 mM N.D. +0.3 -0.1 -0.6 -0.1
motility inner cut, LB soft agar motility assay N.D. +0.4 -1.0 -0.1 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 -0.5 -0.3 +0.3
stress Cobalt chloride 0.3 mM N.D. +0.6 -0.0 -0.1 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.3 -0.6 +0.0 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.5 -0.9 +0.6 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.3 -0.6 +0.4 +0.1
stress 2-pentyl-furan 1.335528 mM N.D. -0.2 +0.6 -0.3 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.2 -0.8 +0.4 +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.3 +0.2 +0.7 -0.6
nitrogen source L-Isoleucine (N) N.D. +0.2 +0.6 -0.1 -0.1
carbon source Glycerol (C) N.D. -0.2 +0.5 +0.5 +0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.1 +0.5 +0.7 -0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.1 +0.3 +0.8 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.2 +0.5 +0.7 -0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.3 +0.5 +0.9 -0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.4 +0.5 +0.7 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.2 +0.4 +0.8 +0.1
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -0.0 +0.8 +0.5 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.4 +0.5 +0.9 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.3 +0.4 +0.9 +0.1
anaerobic; rich media LB; anaerobic N.D. +0.1 +0.4 +0.7 +0.6
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. +0.3 +0.6 +0.9 +0.4
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