Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10365 and AZOBR_RS10370 are separated by 252 nucleotidesAZOBR_RS10370 and AZOBR_RS10375 are separated by 380 nucleotidesAZOBR_RS10375 and AZOBR_RS10380 are separated by 87 nucleotidesAZOBR_RS10380 and AZOBR_RS10385 are separated by 133 nucleotides AZOBR_RS10365: AZOBR_RS10365 - tRNA-Leu, at 2,177,641 to 2,177,727 _RS10365 AZOBR_RS10370: AZOBR_RS10370 - hypothetical protein, at 2,177,980 to 2,180,157 _RS10370 AZOBR_RS10375: AZOBR_RS10375 - glyceraldehyde-3-phosphate dehydrogenase, at 2,180,538 to 2,181,545 _RS10375 AZOBR_RS10380: AZOBR_RS10380 - HicB, at 2,181,633 to 2,181,977 _RS10380 AZOBR_RS10385: AZOBR_RS10385 - hypothetical protein, at 2,182,111 to 2,182,554 _RS10385
Group Condition AZOBR_RS10365 AZOBR_RS10370 AZOBR_RS10375 AZOBR_RS10380 AZOBR_RS10385
stress 2-pentyl-furan 2.671 mM N.D. -0.2 -4.6 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 -1.2 -1.3 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. +0.1 -0.1 -2.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 -0.3 -1.8 N.D.
stress Cobalt chloride 0.2 mM N.D. -0.4 -0.7 -1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 -1.0 -0.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.1 -1.4 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 -0.9 -0.8 N.D.
motility inner cut, LB soft agar motility assay N.D. +0.0 -0.9 -0.9 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.1 -0.6 -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 -0.5 -0.4 N.D.
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM N.D. +0.0 -0.3 -1.1 N.D.
nitrogen source L-Glutamic (N) N.D. +0.1 -1.1 -0.4 N.D.
stress Cobalt chloride 0.3 mM N.D. -0.1 -0.7 -0.5 N.D.
stress Paraquat dichloride 0.02 mg/ml N.D. -0.1 -1.1 -0.1 N.D.
lb LB N.D. -0.5 -0.9 +0.1 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. +0.0 -0.1 -1.1 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. -0.2 +0.5 -1.6 N.D.
stress 1-octen-3-ol 0.6030326667 mM N.D. -0.1 -0.9 -0.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.0 -0.2 -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.9 -0.4 +0.2 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.1 +0.2 -1.4 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.1 -0.6 +0.6 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.1 +0.7 -0.8 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. +0.1 -1.3 +1.5 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.2 -0.3 +0.6 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.3 +0.7 -0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.4 +0.7 -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.5 +0.9 +1.0 N.D.
stress Benzethonium chloride 0.003 mM N.D. +0.3 +0.7 +0.5 N.D.
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