Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10335 and AZOBR_RS10340 are separated by 26 nucleotidesAZOBR_RS10340 and AZOBR_RS10345 overlap by 4 nucleotidesAZOBR_RS10345 and AZOBR_RS10355 are separated by 1550 nucleotidesAZOBR_RS10355 and AZOBR_RS10360 are separated by 107 nucleotides AZOBR_RS10335: AZOBR_RS10335 - acyltransferase, at 2,171,476 to 2,172,294 _RS10335 AZOBR_RS10340: AZOBR_RS10340 - phytoene desaturase, at 2,172,321 to 2,173,862 _RS10340 AZOBR_RS10345: AZOBR_RS10345 - phytoene desaturase, at 2,173,859 to 2,175,412 _RS10345 AZOBR_RS10355: AZOBR_RS10355 - membrane protein, at 2,176,963 to 2,177,427 _RS10355 AZOBR_RS10360: AZOBR_RS10360 - tRNA-Ala, at 2,177,535 to 2,177,610 _RS10360
Group Condition AZOBR_RS10335 AZOBR_RS10340 AZOBR_RS10345 AZOBR_RS10355 AZOBR_RS10360
stress Paraquat dichloride 0.04 mg/ml -2.9 +0.1 -0.3 -0.5 N.D.
lb LB -3.1 -0.3 -0.2 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.3 -1.2 -1.4 N.D.
nitrogen source L-Threonine (N) -0.6 -0.4 -0.3 -2.1 N.D.
stress Sisomicin 0.01 mg/ml -1.7 -0.2 -0.6 -0.7 N.D.
nitrogen source L-Isoleucine (N) -0.9 -0.1 +0.1 -2.1 N.D.
nitrogen source Cytosine (N) -0.9 -0.4 -0.5 -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.6 -0.5 -1.6 N.D.
carbon source Glycerol (C) -0.7 -0.3 -0.3 -1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.4 -0.5 -1.0 N.D.
carbon source D-Lactate (C) -1.0 -0.2 -0.1 -1.0 N.D.
nitrogen source Glucuronamide (N) -0.2 -0.3 -0.2 -1.5 N.D.
stress Chlorite 1 mM +0.0 -0.5 -0.4 -1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.5 -0.3 -1.8 N.D.
nitrogen source nitrate (N) -1.3 -0.2 -0.1 -0.5 N.D.
stress Cobalt chloride 0.3 mM -1.3 +0.0 -0.0 -0.8 N.D.
nitrogen source casaminos (N) -0.8 -0.4 -0.2 -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 -0.6 -0.8 +0.4 N.D.
nitrogen source L-Glutamic (N) +0.5 +0.0 -0.5 -1.0 N.D.
nitrogen source L-Histidine (N) +0.6 -0.4 -0.2 -1.0 N.D.
carbon source succinate (C) +0.6 -0.4 -0.2 -0.9 N.D.
nitrogen source L-Phenylalanine (N) +1.1 -0.3 -0.1 -0.7 N.D.
stress 2-pentyl-furan 1.335528 mM +0.5 +0.7 +0.1 -1.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.5 -0.3 +0.5 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.6 -0.1 +0.2 +0.9 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.3 +0.4 +0.6 +0.6 N.D.
motility inner cut, LB soft agar motility assay +1.2 +0.3 +0.1 +0.2 N.D.
motility outer cut, LB soft agar motility assay +1.5 +0.2 +0.1 +0.3 N.D.
stress D-Cycloserine 0.125 mg/ml +1.4 +0.2 +0.2 +0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.7 +0.0 +0.3 +1.2 N.D.
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