Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10235 and AZOBR_RS10240 are separated by 67 nucleotidesAZOBR_RS10240 and AZOBR_RS10245 are separated by 134 nucleotidesAZOBR_RS10245 and AZOBR_RS10250 are separated by 268 nucleotidesAZOBR_RS10250 and AZOBR_RS10255 overlap by 26 nucleotides AZOBR_RS10235: AZOBR_RS10235 - cobalamin biosynthesis protein CobD, at 2,138,403 to 2,139,365 _RS10235 AZOBR_RS10240: AZOBR_RS10240 - L-threonine-O-3-phosphate decarboxylase, at 2,139,433 to 2,140,452 _RS10240 AZOBR_RS10245: AZOBR_RS10245 - diguanylate cyclase, at 2,140,587 to 2,142,209 _RS10245 AZOBR_RS10250: AZOBR_RS10250 - chemotaxis protein, at 2,142,478 to 2,143,029 _RS10250 AZOBR_RS10255: AZOBR_RS10255 - hypothetical protein, at 2,143,004 to 2,144,311 _RS10255
Group Condition AZOBR_RS10235 AZOBR_RS10240 AZOBR_RS10245 AZOBR_RS10250 AZOBR_RS10255
stress 2-pentyl-furan 1.780666667 mM -3.8 -0.9 +0.0 -0.1 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -1.4 -0.9 -0.3 -0.4 -0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -1.6 -1.3 -0.5 -0.1 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -1.1 -1.0 -0.5 -0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.2 -0.1 +0.1 -0.1 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -1.4 -0.4 -0.4 -0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.7 +0.1 +0.3 -0.1 +0.1
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -1.0 -0.7 -0.2 -0.3 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.4 +0.6 +0.5 -0.3 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.7 +0.1 +0.1 +0.0 -0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -1.0 -0.5 -0.4 -0.1 +0.1
stress Cobalt chloride 0.2 mM -1.6 -0.1 +0.0 -0.2 +0.3
stress 2-pentyl-furan 1.335528 mM -1.2 +0.1 -0.3 -0.2 +0.2
stress 2-pentyl-furan 1.780666667 mM +0.5 -0.8 -0.3 +0.2 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.5 -0.7 -0.1 +0.1 -0.6
stress 2-pentyl-furan 1.335528 mM +0.3 -0.5 +0.4 -0.1 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +1.1 -1.1 +0.1 -0.2 -0.6
lb LB -1.6 +0.9 +0.2 +0.1 +0.3
stress Benzethonium chloride 0.003 mM +1.0 -1.1 -0.1 +0.1 +0.3
motility inner cut, LB soft agar motility assay -1.5 -0.5 +0.3 +0.5 +1.5
nitrogen source L-Glutamic (N) +0.8 -0.3 -0.4 +0.7 -0.3
stress 2-pentyl-furan 2.671 mM +1.1 +0.4 +0.2 -0.2 -0.4
motility inner cut, LB soft agar motility assay -0.9 -0.9 +0.3 +1.4 +1.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +1.2 +0.2 +0.2 +0.3 -0.2
carbon source L-Leucine (C) +1.0 +0.5 -0.1 +0.3 +0.3
carbon source D-Gluconic Acid (C) +0.8 +0.6 +0.0 +0.2 +0.6
motility inner cut, LB soft agar motility assay +0.6 +0.4 +0.2 +1.3 +0.1
nitrogen source casaminos (N) +1.2 +0.8 -0.1 +0.2 +0.9
nitrogen source L-Isoleucine (N) +1.8 +0.5 -0.2 +0.6 +0.6
motility outer cut, LB soft agar motility assay +0.3 -0.6 +1.4 +2.1 +1.0
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