Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10230 and AZOBR_RS10235 are separated by 43 nucleotidesAZOBR_RS10235 and AZOBR_RS10240 are separated by 67 nucleotidesAZOBR_RS10240 and AZOBR_RS10245 are separated by 134 nucleotidesAZOBR_RS10245 and AZOBR_RS10250 are separated by 268 nucleotides AZOBR_RS10230: AZOBR_RS10230 - homospermidine synthase, at 2,136,941 to 2,138,359 _RS10230 AZOBR_RS10235: AZOBR_RS10235 - cobalamin biosynthesis protein CobD, at 2,138,403 to 2,139,365 _RS10235 AZOBR_RS10240: AZOBR_RS10240 - L-threonine-O-3-phosphate decarboxylase, at 2,139,433 to 2,140,452 _RS10240 AZOBR_RS10245: AZOBR_RS10245 - diguanylate cyclase, at 2,140,587 to 2,142,209 _RS10245 AZOBR_RS10250: AZOBR_RS10250 - chemotaxis protein, at 2,142,478 to 2,143,029 _RS10250
Group Condition AZOBR_RS10230 AZOBR_RS10235 AZOBR_RS10240 AZOBR_RS10245 AZOBR_RS10250
stress 2-pentyl-furan 1.780666667 mM +0.2 -3.8 -0.9 +0.0 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -1.1 -1.4 -0.9 -0.3 -0.4
carbon source D-Gluconic Acid (C) -2.7 -0.9 -0.1 -0.1 +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.1 -1.6 -1.3 -0.5 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.9 -1.4 -0.4 -0.4 -0.2
stress Cobalt chloride 0.2 mM -1.3 -1.6 -0.1 +0.0 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -2.2 -0.1 +0.1 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.3 -1.1 -1.0 -0.5 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -2.7 +0.1 +0.3 -0.1
stress Cobalt chloride 0.3 mM -2.2 -0.6 +0.4 +0.0 +0.1
carbon source Glycerol (C) -1.6 -0.3 -0.1 -0.2 +0.3
carbon source L-Glutamine (C) -1.4 -1.1 +0.3 +0.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -2.4 +0.6 +0.5 -0.3
carbon source a-Ketoglutaric (C) -2.0 +0.1 -0.2 +0.1 +0.2
motility inner cut, LB soft agar motility assay -0.3 -1.5 -0.5 +0.3 +0.5
lb LB -0.8 -1.6 +0.9 +0.2 +0.1
carbon source D-Gluconic Acid (C) -2.5 +0.8 +0.6 +0.0 +0.2
nitrogen source L-Glutamic (N) -1.6 +0.8 -0.3 -0.4 +0.7
carbon source D-Fructose (C) -1.8 +0.4 +0.3 +0.1 +0.2
carbon source a-Ketoglutaric (C) -1.7 +0.6 -0.1 +0.3 +0.3
carbon source Glycerol (C) -1.7 +0.5 +0.5 -0.1 +0.3
stress Benzethonium chloride 0.003 mM -0.4 +1.0 -1.1 -0.1 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.2 +1.1 -1.1 +0.1 -0.2
motility inner cut, LB soft agar motility assay +0.4 -0.9 -0.9 +0.3 +1.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +1.5 -0.7 -0.1 -0.1 -0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +1.6 -0.1 -0.5 -0.4 -0.2
carbon source L-Leucine (C) +0.6 +1.0 +0.5 -0.1 +0.3
motility inner cut, LB soft agar motility assay -0.1 +0.6 +0.4 +0.2 +1.3
nitrogen source L-Isoleucine (N) +1.0 +1.8 +0.5 -0.2 +0.6
motility outer cut, LB soft agar motility assay +0.7 +0.3 -0.6 +1.4 +2.1
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