Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS10215 and AZOBR_RS10220 are separated by 132 nucleotidesAZOBR_RS10220 and AZOBR_RS10225 are separated by 421 nucleotidesAZOBR_RS10225 and AZOBR_RS10230 are separated by 140 nucleotidesAZOBR_RS10230 and AZOBR_RS10235 are separated by 43 nucleotides AZOBR_RS10215: AZOBR_RS10215 - acireductone dioxygenase, at 2,133,584 to 2,134,147 _RS10215 AZOBR_RS10220: AZOBR_RS10220 - hypothetical protein, at 2,134,280 to 2,135,104 _RS10220 AZOBR_RS10225: AZOBR_RS10225 - diaminopimelate decarboxylase, at 2,135,526 to 2,136,800 _RS10225 AZOBR_RS10230: AZOBR_RS10230 - homospermidine synthase, at 2,136,941 to 2,138,359 _RS10230 AZOBR_RS10235: AZOBR_RS10235 - cobalamin biosynthesis protein CobD, at 2,138,403 to 2,139,365 _RS10235
Group Condition AZOBR_RS10215 AZOBR_RS10220 AZOBR_RS10225 AZOBR_RS10230 AZOBR_RS10235
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -4.1 -0.1 +0.5 -0.2 -2.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.7 -0.5 +0.2 -0.6 -2.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -4.0 -0.0 -0.0 -0.1 -0.1
stress Cobalt chloride 0.2 mM -0.8 -0.0 -0.3 -1.3 -1.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.6 -0.3 -0.0 -0.3 -1.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -4.1 -0.1 +0.0 -0.3 +0.6
stress 2-pentyl-furan 1.780666667 mM +0.4 +0.1 -0.3 +0.2 -3.8
lb LB -1.2 +0.2 +0.1 -0.8 -1.6
stress D-Cycloserine 0.125 mg/ml -1.6 -0.2 -0.1 -1.1 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.2 -0.2 -0.1 -1.1 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +0.2 +0.3 +0.0 -2.7
stress Chlorite 1 mM -2.1 -0.1 +0.1 -0.4 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.4 +0.0 +0.2 -0.9 -1.4
stress Cobalt chloride 0.3 mM +0.1 +0.3 +0.0 -2.2 -0.6
carbon source D-Gluconic Acid (C) +1.4 +0.1 -0.2 -2.7 -0.9
carbon source L-Glutamine (C) +0.3 +0.1 -0.2 -1.4 -1.1
stress 3-octanone 5.2896 mM -1.7 -0.5 -0.0 +0.2 -0.2
motility inner cut, LB soft agar motility assay -0.6 -0.0 +0.3 -0.3 -1.5
motility inner cut, LB soft agar motility assay -1.5 +0.0 +0.2 +0.4 -0.9
carbon source a-Ketoglutaric (C) +0.4 +0.2 +0.1 -2.0 +0.1
carbon source D-Gluconic Acid (C) +0.7 +0.1 -0.0 -2.5 +0.8
carbon source Glycerol (C) +0.6 -0.3 +0.0 -1.7 +0.5
nitrogen source nitrate (N) +0.7 +0.2 +0.1 -0.6 -1.1
carbon source a-Ketoglutaric (C) +0.3 +0.3 +0.1 -1.7 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -2.1 +0.3 +0.3 +0.2 +1.1
carbon source D-Fructose (C) +0.7 +0.4 +0.1 -1.8 +0.4
nitrogen source L-Glutamic (N) +1.0 +0.2 +0.1 -1.6 +0.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.3 +0.0 +0.3 +1.5 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.5 +0.4 +0.3 -0.3 +0.6
nitrogen source L-Isoleucine (N) +1.0 -0.3 +0.3 +1.0 +1.8
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