Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS09935 and AZOBR_RS09940 are separated by 62 nucleotidesAZOBR_RS09940 and AZOBR_RS09945 are separated by 109 nucleotidesAZOBR_RS09945 and AZOBR_RS09950 are separated by 109 nucleotidesAZOBR_RS09950 and AZOBR_RS09955 are separated by 107 nucleotides AZOBR_RS09935: AZOBR_RS09935 - hypothetical protein, at 2,086,565 to 2,087,263 _RS09935 AZOBR_RS09940: AZOBR_RS09940 - 5'-methylthioadenosine phosphorylase, at 2,087,326 to 2,088,210 _RS09940 AZOBR_RS09945: AZOBR_RS09945 - hypothetical protein, at 2,088,320 to 2,088,733 _RS09945 AZOBR_RS09950: AZOBR_RS09950 - pseudouridine-5'-phosphate glycosidase, at 2,088,843 to 2,089,763 _RS09950 AZOBR_RS09955: AZOBR_RS09955 - beta-lactamase, at 2,089,871 to 2,090,899 _RS09955
Group Condition AZOBR_RS09935 AZOBR_RS09940 AZOBR_RS09945 AZOBR_RS09950 AZOBR_RS09955
nitrogen source L-Isoleucine (N) -5.1 +0.1 N.D. -0.3 -0.2
motility inner cut, LB soft agar motility assay -1.6 -0.2 N.D. -1.0 -0.5
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -2.3 +0.1 N.D. -0.1 +0.0
motility inner cut, LB soft agar motility assay -2.0 -0.2 N.D. +0.1 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.5 -0.4 N.D. +0.0 -0.0
stress 2-pentyl-furan 1.780666667 mM -0.5 -0.2 N.D. -0.3 -0.6
motility inner cut, LB soft agar motility assay -2.2 -0.1 N.D. +0.4 +0.3
stress Cobalt chloride 0.2 mM -1.6 +0.0 N.D. +0.2 -0.0
stress 2-pentyl-furan 1.335528 mM -0.9 +0.2 N.D. -0.2 -0.5
stress 2-pentyl-furan 1.335528 mM -0.6 -0.2 N.D. -0.3 -0.3
stress Sisomicin 0.01 mg/ml -1.6 +0.3 N.D. +0.2 +0.3
lb LB -0.8 +0.1 N.D. +0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 -0.3 N.D. +0.8 +0.5
motility outer cut, LB soft agar motility assay +0.6 -0.8 N.D. +0.4 -0.4
stress 2-pentyl-furan 2.671 mM +0.8 -0.3 N.D. -0.5 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.7 +0.4 N.D. +0.2 +0.2
carbon source D-Gluconic Acid (C) +0.8 -0.7 N.D. +0.4 -0.2
nitrogen source L-Aspartic Acid (N) +0.7 -0.1 N.D. +0.2 -0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 +0.3 N.D. +0.6 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.5 N.D. +0.2 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 -0.3 N.D. +0.3 -0.1
nitrogen source L-Leucine (N) +1.0 -0.4 N.D. +0.1 +0.2
nitrogen source L-Serine (N) +0.5 -0.2 N.D. +0.4 +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.3 +0.4 N.D. -0.4 +0.5
stress D-Cycloserine 0.125 mg/ml +1.1 -0.4 N.D. +0.2 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.2 N.D. +0.5 +0.2
stress 3-octanone 6.3476 mM +0.9 -0.2 N.D. +0.2 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 +0.2 N.D. +0.3 +0.2
nitrogen source casaminos (N) +1.0 +0.4 N.D. +0.2 +0.2
stress Benzethonium chloride 0.003 mM +1.5 +0.0 N.D. +0.0 +0.3
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