Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS09875 and AZOBR_RS09880 overlap by 29 nucleotidesAZOBR_RS09880 and AZOBR_RS09885 are separated by 201 nucleotidesAZOBR_RS09885 and AZOBR_RS09890 are separated by 24 nucleotidesAZOBR_RS09890 and AZOBR_RS09895 are separated by 287 nucleotides AZOBR_RS09875: AZOBR_RS09875 - membrane protein, at 2,069,505 to 2,070,044 _RS09875 AZOBR_RS09880: AZOBR_RS09880 - hypothetical protein, at 2,070,016 to 2,070,390 _RS09880 AZOBR_RS09885: AZOBR_RS09885 - Crp/Fnr family transcriptional regulator, at 2,070,592 to 2,071,299 _RS09885 AZOBR_RS09890: AZOBR_RS09890 - hypothetical protein, at 2,071,324 to 2,071,737 _RS09890 AZOBR_RS09895: AZOBR_RS09895 - hypothetical protein, at 2,072,025 to 2,072,669 _RS09895
Group Condition AZOBR_RS09875 AZOBR_RS09880 AZOBR_RS09885 AZOBR_RS09890 AZOBR_RS09895
motility inner cut, LB soft agar motility assay -0.2 -0.6 N.D. -0.1 -3.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.8 N.D. -0.4 -2.8
stress 3-octanone 6.3476 mM -0.5 -0.6 N.D. -0.6 -1.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 -0.3 N.D. +0.6 -2.8
stress Cobalt chloride 0.3 mM -0.3 +0.3 N.D. -1.6 -0.4
stress 3-octanone 6.3476 mM +0.0 -0.1 N.D. -0.2 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.0 N.D. -0.9 -0.7
nitrogen source L-Aspartic Acid (N) -0.2 +0.1 N.D. -0.1 -1.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 +0.1 N.D. -0.4 -1.0
stress Paraquat dichloride 0.02 mg/ml -0.2 +0.3 N.D. -0.3 -1.3
stress Sisomicin 0.01 mg/ml -0.2 +0.3 N.D. -1.0 -0.5
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.3 -0.5 N.D. -0.8 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -1.7 N.D. -0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 -0.4 N.D. +0.1 -1.1
stress 2-pentyl-furan 1.780666667 mM +0.3 +0.3 N.D. -0.4 -1.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 -0.3 N.D. -1.0 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.2 -0.3 N.D. -0.7 +0.4
stress 2-pentyl-furan 2.671 mM -0.5 +0.1 N.D. -0.6 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.1 -0.0 N.D. -1.0 +0.7
stress 2-pentyl-furan 1.780666667 mM -0.2 +0.2 N.D. -0.9 +0.6
nitrogen source casaminos (N) -0.1 +0.6 N.D. +0.3 -0.9
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.1 -0.1 N.D. -0.7 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.1 N.D. +1.0 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.5 N.D. -0.1 +1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.1 N.D. +1.2 -0.7
nitrogen source L-Leucine (N) -0.2 +0.1 N.D. +0.7 +0.8
carbon source Glycerol (C) +0.1 +0.0 N.D. +0.8 +0.7
carbon source a-Ketoglutaric (C) +0.2 -0.1 N.D. +0.9 +1.0
nitrogen source L-Glutamic (N) +0.3 +0.5 N.D. +0.8 +0.8
nitrogen source L-Isoleucine (N) +0.7 +0.0 N.D. +2.0 +0.3
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