Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS09720 and AZOBR_RS09725 are separated by 139 nucleotidesAZOBR_RS09725 and AZOBR_RS09730 are separated by 141 nucleotidesAZOBR_RS09730 and AZOBR_RS09735 are separated by 217 nucleotidesAZOBR_RS09735 and AZOBR_RS09740 are separated by 61 nucleotides AZOBR_RS09720: AZOBR_RS09720 - succinate-semialdehyde dehdyrogenase, at 2,035,614 to 2,037,107 _RS09720 AZOBR_RS09725: AZOBR_RS09725 - hypothetical protein, at 2,037,247 to 2,037,699 _RS09725 AZOBR_RS09730: AZOBR_RS09730 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 2,037,841 to 2,039,226 _RS09730 AZOBR_RS09735: AZOBR_RS09735 - MFS transporter permease, at 2,039,444 to 2,040,793 _RS09735 AZOBR_RS09740: AZOBR_RS09740 - Mn-containing catalase, at 2,040,855 to 2,041,751 _RS09740
Group Condition AZOBR_RS09720 AZOBR_RS09725 AZOBR_RS09730 AZOBR_RS09735 AZOBR_RS09740
lb LB -0.5 -1.5 N.D. -0.8 -0.0
anaerobic; rich media LB; anaerobic -0.0 -0.3 N.D. -2.4 +0.2
stress Sisomicin 0.01 mg/ml -1.1 +0.3 N.D. -1.0 -0.2
stress Bacitracin 0.25 mg/ml +0.1 +0.1 N.D. -2.0 -0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.4 -0.3 N.D. -1.2 -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.1 -0.7 N.D. -1.0 -0.1
stress Benzethonium chloride 0.003 mM +0.2 -0.6 N.D. -1.3 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.4 -0.3 N.D. -0.9 -0.2
motility inner cut, LB soft agar motility assay -0.4 -0.2 N.D. -1.3 +0.1
motility outer cut, LB soft agar motility assay -0.2 -0.7 N.D. -1.1 +0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 -0.1 N.D. -1.3 -0.1
motility inner cut, LB soft agar motility assay -0.3 +0.1 N.D. -1.4 +0.1
nitrogen source D-Alanine (N) +0.1 -0.4 N.D. -1.0 -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.0 -0.5 N.D. -0.8 -0.1
stress Vancomycin 0.03 mg/ml +0.1 -0.2 N.D. -1.2 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.2 -0.7 N.D. -1.1 +0.3
nitrogen source D-Alanine (N) +0.2 +0.1 N.D. -1.3 -0.0
stress D-Cycloserine 0.125 mg/ml -0.1 -0.6 N.D. -0.5 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.8 N.D. -0.7 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.3 N.D. -0.8 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 +0.4 N.D. -1.1 -0.5
stress 3-octanone 5.2896 mM +0.0 +0.0 N.D. -1.1 +0.3
stress 3-octanone 5.2896 mM +0.3 +0.4 N.D. -1.2 -0.1
stress 3-octanone 5.2896 mM +0.1 +0.3 N.D. -0.8 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.9 N.D. +0.2 -0.1
stress 2-pentyl-furan 1.780666667 mM -0.5 +0.3 N.D. -0.6 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 +0.6 N.D. -0.6 -0.3
stress 3-octanone 6.3476 mM +0.1 +0.3 N.D. -0.9 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +0.1 N.D. -0.4 -0.3
stress 2-Furfuraldehyde 0.2 vol% +0.2 +0.8 N.D. -0.4 +0.2
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