Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS09705 and AZOBR_RS09710 are separated by 231 nucleotidesAZOBR_RS09710 and AZOBR_RS09715 are separated by 187 nucleotidesAZOBR_RS09715 and AZOBR_RS09720 are separated by 128 nucleotidesAZOBR_RS09720 and AZOBR_RS09725 are separated by 139 nucleotides AZOBR_RS09705: AZOBR_RS09705 - tRNA-Val, at 2,033,961 to 2,034,035 _RS09705 AZOBR_RS09710: AZOBR_RS09710 - sugar tyrosine-protein kinase, at 2,034,267 to 2,034,431 _RS09710 AZOBR_RS09715: AZOBR_RS09715 - pantoate--beta-alanine ligase, at 2,034,619 to 2,035,485 _RS09715 AZOBR_RS09720: AZOBR_RS09720 - succinate-semialdehyde dehdyrogenase, at 2,035,614 to 2,037,107 _RS09720 AZOBR_RS09725: AZOBR_RS09725 - hypothetical protein, at 2,037,247 to 2,037,699 _RS09725
Group Condition AZOBR_RS09705 AZOBR_RS09710 AZOBR_RS09715 AZOBR_RS09720 AZOBR_RS09725
lb LB N.D. N.D. +0.0 -0.5 -1.5
carbon source D-Gluconic Acid (C) N.D. N.D. -1.5 +0.2 -0.2
nitrogen source L-Phenylalanine (N) N.D. N.D. -0.5 +0.0 -0.7
stress D-Cycloserine 0.125 mg/ml N.D. N.D. -0.4 -0.1 -0.6
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. N.D. -0.5 -0.2 -0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. N.D. -0.2 -0.1 -0.7
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. N.D. -0.3 -0.4 -0.3
carbon source D-Fructose (C) N.D. N.D. -0.7 +0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.0 +0.2 -0.9
carbon source D-Gluconic Acid (C) N.D. N.D. -0.5 +0.2 -0.3
stress Sisomicin 0.01 mg/ml N.D. N.D. +0.2 -1.1 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.0 +0.2 -0.7
nitrogen source L-Leucine (N) N.D. N.D. -0.6 +0.3 -0.2
motility outer cut, LB soft agar motility assay N.D. N.D. +0.5 -0.2 -0.7
stress Benzethonium chloride 0.003 mM N.D. N.D. +0.1 +0.2 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.5 +0.1 -0.8
stress 3-octanone 6.3476 mM N.D. N.D. -0.6 +0.2 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.4 -0.4 +0.3
stress 3-octanone 5.2896 mM N.D. N.D. -0.4 +0.3 +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. N.D. -0.4 +0.2 +0.4
lb no stress control LB no stress control N.D. N.D. +0.3 -0.1 +0.5
stress 2-pentyl-furan 1.335528 mM N.D. N.D. +0.8 -0.3 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.4 +0.0 +0.6
stress 2-Furfuraldehyde 0.2 vol% N.D. N.D. +0.1 +0.2 +0.8
lb LB N.D. N.D. +0.9 +0.1 +0.0
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM N.D. N.D. +0.4 +0.1 +0.5
lb no stress control LB no stress control N.D. N.D. +0.6 +0.2 +0.5
stress 2-pentyl-furan 2.671 mM N.D. N.D. +0.8 -0.1 +0.5
stress 3-octanone 6.3476 mM N.D. N.D. +0.7 +0.0 +0.5
stress 2-pentyl-furan 1.335528 mM N.D. N.D. +0.7 +0.2 +0.4
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