Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS09580 and AZOBR_RS09585 are separated by 519 nucleotidesAZOBR_RS09585 and AZOBR_RS09590 are separated by 119 nucleotidesAZOBR_RS09590 and AZOBR_RS09595 are separated by 92 nucleotidesAZOBR_RS09595 and AZOBR_RS09600 overlap by 4 nucleotides AZOBR_RS09580: AZOBR_RS09580 - exported protein of unknown function, at 2,008,560 to 2,009,315 _RS09580 AZOBR_RS09585: AZOBR_RS09585 - hypothetical protein, at 2,009,835 to 2,010,989 _RS09585 AZOBR_RS09590: AZOBR_RS09590 - putative Zn-ribbon-containing protein involved in phosphonate metabolism PhnA-like protein, at 2,011,109 to 2,011,465 _RS09590 AZOBR_RS09595: AZOBR_RS09595 - C4-dicarboxylate ABC transporter permease, at 2,011,558 to 2,012,856 _RS09595 AZOBR_RS09600: AZOBR_RS09600 - C4-dicarboxylate ABC transporter permease, at 2,012,853 to 2,013,533 _RS09600
Group Condition AZOBR_RS09580 AZOBR_RS09585 AZOBR_RS09590 AZOBR_RS09595 AZOBR_RS09600
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -1.6 -3.0 -0.3 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 +0.3 -3.0 +0.1 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -0.9 -1.0 -0.4 -0.7
carbon source a-Ketoglutaric (C) +0.0 -0.5 -0.5 -0.9 -1.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.0 -0.9 -0.5 -0.9 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.3 -3.0 -0.1 +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.6 -0.5 -2.4 +0.0 -0.3
stress 3-octanone 6.3476 mM -0.8 -1.5 +0.1 -0.2 +0.0
stress Paraquat dichloride 0.04 mg/ml +0.5 -1.7 -1.4 +0.2 +0.2
motility outer cut, LB soft agar motility assay -0.0 -0.3 +0.3 +0.0 -1.7
nitrogen source L-Isoleucine (N) -0.5 +0.2 +0.3 -0.3 -1.3
nitrogen source casaminos (N) -0.2 -0.5 -1.1 +0.4 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 -0.0 -1.7 -0.3 +0.5
stress Paraquat dichloride 0.02 mg/ml +0.1 -0.5 -1.4 +0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +0.4 -2.1 +0.3 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.0 -0.2 +0.4 -1.0 -0.6
stress Cobalt chloride 0.3 mM -0.0 -0.6 -0.5 +0.6 -0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.0 -0.0 +0.5 -1.1 -0.6
stress Chlorite 1 mM -0.2 -0.4 -1.2 +0.2 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.6 -1.9 +0.5 -0.4
stress Cobalt chloride 0.2 mM +0.3 -0.3 -1.1 +0.3 +0.4
nitrogen source L-Threonine (N) +0.3 +0.8 -0.8 -0.0 -0.6
motility inner cut, LB soft agar motility assay +0.8 -1.4 +0.4 +0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.7 -2.0 -0.0 +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.6 -0.5 +1.8 -0.5 -0.5
carbon source D-Gluconic Acid (C) +0.3 +0.9 +0.8 -0.3 -1.4
stress 3-octanone 6.3476 mM +0.1 +1.0 -0.8 +0.2 +0.5
stress 2-pentyl-furan 2.671 mM -0.1 -0.7 +0.9 +0.2 +0.7
stress Sisomicin 0.01 mg/ml -0.0 +0.0 +1.8 -0.2 -0.3
stress D-Cycloserine 0.125 mg/ml +0.6 -0.5 +1.4 -0.2 +0.2
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