Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS09500 and AZOBR_RS09505 are separated by 98 nucleotidesAZOBR_RS09505 and AZOBR_RS09510 are separated by 34 nucleotidesAZOBR_RS09510 and AZOBR_RS09515 are separated by 123 nucleotidesAZOBR_RS09515 and AZOBR_RS09520 are separated by 54 nucleotides AZOBR_RS09500: AZOBR_RS09500 - hypothetical protein, at 1,988,694 to 1,989,377 _RS09500 AZOBR_RS09505: AZOBR_RS09505 - DNA polymerase III epsilon subunit, at 1,989,476 to 1,990,789 _RS09505 AZOBR_RS09510: AZOBR_RS09510 - hypothetical protein, at 1,990,824 to 1,991,291 _RS09510 AZOBR_RS09515: AZOBR_RS09515 - heme ABC transporter ATP-binding protein, at 1,991,415 to 1,993,091 _RS09515 AZOBR_RS09520: AZOBR_RS09520 - hypothetical protein, at 1,993,146 to 1,994,627 _RS09520
Group Condition AZOBR_RS09500 AZOBR_RS09505 AZOBR_RS09510 AZOBR_RS09515 AZOBR_RS09520
motility outer cut, LB soft agar motility assay -1.0 -2.3 N.D. -2.4 +1.0
stress 2-pentyl-furan 1.780666667 mM -0.4 -1.1 N.D. -1.5 -1.0
stress 2-pentyl-furan 1.780666667 mM +0.4 -1.0 N.D. -1.5 -0.2
stress 2-pentyl-furan 1.780666667 mM -0.2 -0.8 N.D. -1.4 +0.4
stress 2-pentyl-furan 1.335528 mM +0.1 -1.5 N.D. -0.3 -0.3
stress Cobalt chloride 0.2 mM -0.4 -0.3 N.D. -1.4 +0.2
stress D-Cycloserine 0.125 mg/ml +0.3 +0.3 N.D. -1.2 -1.2
stress 2-pentyl-furan 1.335528 mM -0.1 -1.0 N.D. -0.6 -0.0
stress Paraquat dichloride 0.02 mg/ml +0.1 -0.4 N.D. -0.8 -0.6
nitrogen source L-Glutamic (N) -0.6 +0.5 N.D. -1.2 -0.3
stress Cobalt chloride 0.3 mM +0.2 -0.3 N.D. -0.8 -0.6
lb LB +0.3 -0.7 N.D. -0.2 -0.8
motility inner cut, LB soft agar motility assay -0.1 +0.4 N.D. -1.3 -0.4
motility inner cut, LB soft agar motility assay -0.3 -0.3 N.D. -1.1 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.0 +0.6 N.D. -0.0 -1.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.0 -1.7 N.D. +1.2 +0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.4 -1.8 N.D. +1.1 +0.4
nitrogen source L-Histidine (N) -0.6 +0.5 N.D. -0.2 +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.2 -0.5 N.D. +0.8 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.2 -1.5 N.D. +1.5 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.6 -1.0 N.D. +1.0 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.1 -0.9 N.D. +0.9 +0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.2 -1.3 N.D. +1.5 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +1.1 N.D. -0.4 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +1.1 N.D. -0.4 -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.2 -0.7 N.D. +1.2 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.8 -1.5 N.D. +1.4 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 +0.6 N.D. +0.3 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.6 N.D. +0.1 +0.9
nitrogen source L-Isoleucine (N) -0.2 -0.4 N.D. +1.5 +1.7
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