Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS09150 and AZOBR_RS09155 are separated by 96 nucleotidesAZOBR_RS09155 and AZOBR_RS09160 are separated by 21 nucleotidesAZOBR_RS09160 and AZOBR_RS09165 are separated by 422 nucleotidesAZOBR_RS09165 and AZOBR_RS09170 are separated by 218 nucleotides AZOBR_RS09150: AZOBR_RS09150 - peroxiredoxin, at 1,911,455 to 1,911,874 _RS09150 AZOBR_RS09155: AZOBR_RS09155 - short-chain dehydrogenase, at 1,911,971 to 1,913,002 _RS09155 AZOBR_RS09160: AZOBR_RS09160 - multidrug transporter, at 1,913,024 to 1,913,341 _RS09160 AZOBR_RS09165: AZOBR_RS09165 - hypothetical protein, at 1,913,764 to 1,914,048 _RS09165 AZOBR_RS09170: AZOBR_RS09170 - exported protein of unknown function, at 1,914,267 to 1,914,839 _RS09170
Group Condition AZOBR_RS09150 AZOBR_RS09155 AZOBR_RS09160 AZOBR_RS09165 AZOBR_RS09170
motility outer cut, LB soft agar motility assay N.D. -0.1 -1.4 -2.4 -0.4
carbon source D-Gluconic Acid (C) N.D. -0.5 -1.6 -1.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.6 +0.1 -0.7 -0.9
lb LB N.D. -0.6 -0.7 -0.8 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.1 -0.4 -0.2 -1.3
carbon source pyruvate (C) N.D. -0.3 -0.4 -1.1 +0.2
carbon source D,L-Lactate (C) N.D. -0.2 -0.8 -1.0 +0.5
nitrogen source L-Threonine (N) N.D. -0.0 +0.3 -0.0 -1.4
stress 2-pentyl-furan 1.780666667 mM N.D. +0.1 +0.9 -1.0 -0.9
nitrogen source L-Citrulline (N) N.D. +0.3 -0.6 -0.7 +0.5
nitrogen source L-Glutamic (N) N.D. -0.0 +0.4 +0.7 -1.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.3 -0.3 +0.8 -0.8
motility inner cut, LB soft agar motility assay N.D. -0.6 -0.2 +1.3 -0.4
stress Cobalt chloride 0.3 mM N.D. +0.1 -0.3 -0.7 +1.2
nitrogen source L-Isoleucine (N) N.D. -0.4 +0.1 -0.2 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.5 +0.3 -0.4 +1.4
stress 2-pentyl-furan 1.335528 mM N.D. +0.2 +0.2 +1.3 -0.8
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM N.D. -0.0 +0.6 +0.9 -0.5
stress 2-pentyl-furan 2.671 mM N.D. +0.5 +0.7 +0.4 -0.5
stress 3-octanone 6.3476 mM N.D. -0.5 +1.3 +0.5 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 +0.2 +0.8 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.1 -0.2 +1.3 +0.3
nitrogen source casaminos (N) N.D. +0.3 -0.2 +0.9 +0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.1 -0.7 +1.4 +0.8
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.6 -0.3 +1.2 +0.2
anaerobic; rich media LB; anaerobic N.D. +0.0 +0.1 +1.1 +0.6
stress 3-octanone 5.2896 mM N.D. -0.0 +0.8 +0.9 +0.2
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. +0.3 +0.1 +1.2 +0.3
stress 3-octanone 6.3476 mM N.D. -0.2 +1.0 +1.3 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.5 +0.1 +1.3 +0.4
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