Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS09130 and AZOBR_RS09135 overlap by 32 nucleotidesAZOBR_RS09135 and AZOBR_RS09140 are separated by 260 nucleotidesAZOBR_RS09140 and AZOBR_RS09145 are separated by 56 nucleotidesAZOBR_RS09145 and AZOBR_RS09150 are separated by 13 nucleotides AZOBR_RS09130: AZOBR_RS09130 - conserved membrane protein of unknown function, at 1,908,008 to 1,908,946 _RS09130 AZOBR_RS09135: AZOBR_RS09135 - GCN5 family acetyltransferase, at 1,908,915 to 1,909,355 _RS09135 AZOBR_RS09140: AZOBR_RS09140 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 1,909,616 to 1,910,920 _RS09140 AZOBR_RS09145: AZOBR_RS09145 - hypothetical protein, at 1,910,977 to 1,911,441 _RS09145 AZOBR_RS09150: AZOBR_RS09150 - peroxiredoxin, at 1,911,455 to 1,911,874 _RS09150
Group Condition AZOBR_RS09130 AZOBR_RS09135 AZOBR_RS09140 AZOBR_RS09145 AZOBR_RS09150
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.6 N.D. N.D. N.D.
stress copper (II) chloride 2 mM +0.0 -0.6 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.3 N.D. N.D. N.D.
lb LB +0.1 -0.5 N.D. N.D. N.D.
nitrogen source D-Alanine (N) -0.3 +0.2 N.D. N.D. N.D.
stress D-Cycloserine 0.125 mg/ml +0.2 -0.3 N.D. N.D. N.D.
stress 2-pentyl-furan 1.335528 mM +0.2 -0.3 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 -0.3 N.D. N.D. N.D.
nitrogen source L-Threonine (N) -0.3 +0.3 N.D. N.D. N.D.
stress Vancomycin 0.03 mg/ml +0.3 -0.2 N.D. N.D. N.D.
stress 2-pentyl-furan 1.335528 mM -0.4 +0.6 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.4 N.D. N.D. N.D.
stress 3-octanone 6.3476 mM -0.3 +0.6 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.5 -0.0 N.D. N.D. N.D.
nitrogen source L-Isoleucine (N) -0.2 +0.7 N.D. N.D. N.D.
stress Benzethonium chloride 0.003 mM +0.3 +0.3 N.D. N.D. N.D.
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM +0.3 +0.3 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.5 +0.1 N.D. N.D. N.D.
carbon source Glycerol (C) +0.5 +0.1 N.D. N.D. N.D.
stress Paraquat dichloride 0.04 mg/ml +0.4 +0.2 N.D. N.D. N.D.
carbon source L-Lactate (C) +0.3 +0.3 N.D. N.D. N.D.
stress 1-octen-3-ol 0.7753276667 mM +0.4 +0.2 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.4 +0.3 N.D. N.D. N.D.
stress 2-pentyl-furan 1.335528 mM +0.4 +0.3 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.5 +0.2 N.D. N.D. N.D.
carbon source L-Malic (C) +0.4 +0.3 N.D. N.D. N.D.
nitrogen source L-Aspartic Acid (N) +0.5 +0.2 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.5 +0.3 N.D. N.D. N.D.
nitrogen source D-Alanine (N) +0.3 +0.5 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.6 +0.4 N.D. N.D. N.D.
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