Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS08965 and AZOBR_RS08970 are separated by 434 nucleotidesAZOBR_RS08970 and AZOBR_RS08975 are separated by 95 nucleotidesAZOBR_RS08975 and AZOBR_RS08980 overlap by 29 nucleotidesAZOBR_RS08980 and AZOBR_RS08985 are separated by 244 nucleotides AZOBR_RS08965: AZOBR_RS08965 - excinuclease ABC subunit C, at 1,874,390 to 1,876,351 _RS08965 AZOBR_RS08970: AZOBR_RS08970 - short-chain dehydrogenase, at 1,876,786 to 1,877,556 _RS08970 AZOBR_RS08975: AZOBR_RS08975 - hypothetical protein, at 1,877,652 to 1,878,452 _RS08975 AZOBR_RS08980: AZOBR_RS08980 - hypothetical protein, at 1,878,424 to 1,878,714 _RS08980 AZOBR_RS08985: AZOBR_RS08985 - 5S ribosomal RNA, at 1,878,959 to 1,879,074 _RS08985
Group Condition AZOBR_RS08965 AZOBR_RS08970 AZOBR_RS08975 AZOBR_RS08980 AZOBR_RS08985
motility inner cut, LB soft agar motility assay -0.1 -1.3 -2.0 -0.0 N.D.
stress Benzethonium chloride 0.003 mM -0.1 -0.5 -1.5 -0.2 N.D.
stress 3-octanone 6.3476 mM -0.1 -0.8 -1.3 +0.2 N.D.
stress 3-octanone 6.3476 mM -0.3 -0.5 -0.8 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.7 +0.1 -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.2 -0.2 -2.0 +0.5 N.D.
stress Cobalt chloride 0.2 mM -0.6 -0.5 -0.1 -0.2 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 +0.0 -1.0 -0.5 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.2 +0.1 -1.1 -0.5 N.D.
lb LB -0.3 -1.7 +0.6 +0.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.1 +0.1 -0.7 -0.4 N.D.
motility inner cut, LB soft agar motility assay -0.4 -0.6 +0.9 -0.5 N.D.
stress Chlorite 1 mM -0.0 -0.5 -0.4 +0.5 N.D.
stress 2-pentyl-furan 2.671 mM -0.4 -0.5 +0.3 +0.3 N.D.
nitrogen source nitrate (N) +0.1 -0.8 +0.5 -0.1 N.D.
stress Vancomycin 0.03 mg/ml -0.3 +0.2 +0.4 -0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -0.6 +0.7 -0.1 N.D.
carbon source D-Gluconic Acid (C) +0.3 -0.4 +0.6 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.7 +1.2 +0.0 N.D.
nitrogen source L-Isoleucine (N) +0.5 +0.3 -1.1 +0.7 N.D.
stress Paraquat dichloride 0.04 mg/ml -0.1 -0.4 +1.1 -0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.6 +1.3 +0.1 N.D.
motility inner cut, LB soft agar motility assay -0.3 -0.4 +0.8 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.4 +1.2 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.6 +1.1 +0.4 N.D.
nitrogen source Glucuronamide (N) +0.0 -0.3 +0.6 +0.4 N.D.
carbon source D-Fructose (C) +0.2 -0.2 +0.9 -0.1 N.D.
carbon source a-Ketoglutaric (C) +0.1 -0.2 +0.7 +0.5 N.D.
carbon source L-Malic (C) +0.1 +0.2 +0.7 +0.3 N.D.
motility outer cut, LB soft agar motility assay -0.1 +0.1 +2.2 +0.3 N.D.
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