Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS08815 and AZOBR_RS08820 are separated by 158 nucleotidesAZOBR_RS08820 and AZOBR_RS08825 are separated by 147 nucleotidesAZOBR_RS08825 and AZOBR_RS08830 are separated by 400 nucleotidesAZOBR_RS08830 and AZOBR_RS08835 overlap by 4 nucleotides AZOBR_RS08815: AZOBR_RS08815 - protein phosphatase, at 1,844,944 to 1,845,723 _RS08815 AZOBR_RS08820: AZOBR_RS08820 - hypothetical protein, at 1,845,882 to 1,846,151 _RS08820 AZOBR_RS08825: AZOBR_RS08825 - agmatine deiminase, at 1,846,299 to 1,847,315 _RS08825 AZOBR_RS08830: AZOBR_RS08830 - putrescine/spermidine ABC transporter permease, at 1,847,716 to 1,848,549 _RS08830 AZOBR_RS08835: AZOBR_RS08835 - putrescine/spermidine ABC transporter permease, at 1,848,546 to 1,849,469 _RS08835
Group Condition AZOBR_RS08815 AZOBR_RS08820 AZOBR_RS08825 AZOBR_RS08830 AZOBR_RS08835
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.5 -2.5 -1.0
stress 2-pentyl-furan 1.335528 mM N.D. N.D. -0.1 -1.9 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.2 -0.9 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. N.D. -1.2 -0.3 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. -0.6 -0.5 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. -0.7 -0.7 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.9 -0.3 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.0 -1.0 -0.5
anaerobic; rich media LB; anaerobic N.D. N.D. -1.1 -0.3 -0.0
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. +0.0 -1.2 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. N.D. -1.1 -0.2 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. -0.5 -0.8 +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. N.D. -0.8 -0.6 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. N.D. -1.1 -0.3 +0.1
stress copper (II) chloride 2 mM N.D. N.D. -0.6 -0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.5 -1.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.5 -0.8 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.2 -1.2 +0.2
carbon source D-Gluconic Acid (C) N.D. N.D. -1.0 +0.6 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.4 -0.2 -0.6
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. +0.5 -0.2 -0.6
motility outer cut, LB soft agar motility assay N.D. N.D. +0.7 -0.3 -0.4
carbon source D-Gluconic Acid (C) N.D. N.D. -0.4 +0.8 +0.0
lb LB N.D. N.D. +0.2 -0.4 +0.8
motility inner cut, LB soft agar motility assay N.D. N.D. +2.0 -1.7 +0.2
carbon source succinate (C) N.D. N.D. +0.7 +0.5 +0.2
nitrogen source L-Isoleucine (N) N.D. N.D. +0.2 +0.9 +0.3
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. +0.6 +0.7 +0.1
stress Nickel (II) chloride 0.5 mM N.D. N.D. +0.3 +0.8 +0.5
stress Paraquat dichloride 0.04 mg/ml N.D. N.D. +0.3 +0.8 +1.1
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