Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS08740 and AZOBR_RS08750 are separated by 1568 nucleotidesAZOBR_RS08750 and AZOBR_RS08755 are separated by 50 nucleotidesAZOBR_RS08755 and AZOBR_RS08760 are separated by 94 nucleotidesAZOBR_RS08760 and AZOBR_RS08765 are separated by 160 nucleotides AZOBR_RS08740: AZOBR_RS08740 - thiosulfate sulfurtransferase, at 1,826,869 to 1,827,195 _RS08740 AZOBR_RS08750: AZOBR_RS08750 - hypothetical protein, at 1,828,764 to 1,829,423 _RS08750 AZOBR_RS08755: AZOBR_RS08755 - valyl-tRNA synthetase, at 1,829,474 to 1,832,131 _RS08755 AZOBR_RS08760: AZOBR_RS08760 - putative methyl-accepting chemotaxis protein, at 1,832,226 to 1,834,184 _RS08760 AZOBR_RS08765: AZOBR_RS08765 - membrane protein, at 1,834,345 to 1,835,178 _RS08765
Group Condition AZOBR_RS08740 AZOBR_RS08750 AZOBR_RS08755 AZOBR_RS08760 AZOBR_RS08765
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -4.9 N.D. -0.3 -0.9
stress 3-octanone 6.3476 mM N.D. -3.2 N.D. -0.4 -1.2
stress 2-pentyl-furan 1.335528 mM N.D. -3.2 N.D. -0.0 -1.1
nitrogen source L-Isoleucine (N) N.D. -4.3 N.D. -0.9 +0.9
stress 2-pentyl-furan 1.335528 mM N.D. -3.1 N.D. -0.7 -0.2
carbon source succinate (C) N.D. -2.7 N.D. -0.8 -0.3
stress 2-pentyl-furan 1.780666667 mM N.D. -3.3 N.D. +0.1 -0.4
nitrogen source Urea (N) N.D. -3.0 N.D. -0.1 -0.2
stress 3-octanone 6.3476 mM N.D. -1.6 N.D. +0.0 -1.8
motility inner cut, LB soft agar motility assay N.D. -3.8 N.D. -0.0 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. -3.6 N.D. +0.3 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -3.6 N.D. +0.3 +0.3
stress 3-octanone 6.3476 mM N.D. -3.0 N.D. -0.3 +0.3
stress 3-octanone 6.3476 mM N.D. -3.3 N.D. +0.4 -0.1
stress 1-octen-3-ol 0.6784116667 mM N.D. -3.3 N.D. +0.3 -0.0
motility inner cut, LB soft agar motility assay N.D. -4.2 N.D. +0.3 +1.0
stress 2-pentyl-furan 1.335528 mM N.D. -1.6 N.D. +0.3 -1.5
nitrogen source Parabanic Acid (N) N.D. -3.1 N.D. -0.1 +0.5
carbon source a-Ketoglutaric (C) N.D. -2.9 N.D. -0.2 +0.4
stress 2-pentyl-furan 1.780666667 mM N.D. -3.3 N.D. +0.1 +0.5
motility inner cut, LB soft agar motility assay N.D. -4.1 N.D. +0.6 +0.8
carbon source L-Glutamine (C) N.D. -2.7 N.D. -0.3 +0.3
nitrogen source Parabanic Acid (N) N.D. -2.9 N.D. -0.3 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. -3.7 N.D. +0.6 +0.5
motility outer cut, LB soft agar motility assay N.D. -4.1 N.D. +0.7 +1.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -2.4 N.D. -0.6 +0.7
carbon source D-Gluconic Acid (C) N.D. -2.4 N.D. -0.7 +1.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -2.8 N.D. +1.3 -0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -2.7 N.D. +1.3 +0.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -2.4 N.D. +0.6 +0.8
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