Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS08670 and AZOBR_RS08675 are separated by 141 nucleotidesAZOBR_RS08675 and AZOBR_RS08680 are separated by 55 nucleotidesAZOBR_RS08680 and AZOBR_RS08685 are separated by 52 nucleotidesAZOBR_RS08685 and AZOBR_RS08690 overlap by 25 nucleotides AZOBR_RS08670: AZOBR_RS08670 - arginine ABC transporter ATP-binding protein, at 1,815,797 to 1,816,573 _RS08670 AZOBR_RS08675: AZOBR_RS08675 - histidinol phosphate aminotransferase, at 1,816,715 to 1,817,800 _RS08675 AZOBR_RS08680: AZOBR_RS08680 - multidrug DMT transporter permease, at 1,817,856 to 1,818,689 _RS08680 AZOBR_RS08685: AZOBR_RS08685 - GNAT family acetyltransferase, at 1,818,742 to 1,819,383 _RS08685 AZOBR_RS08690: AZOBR_RS08690 - acetyltransferase, at 1,819,359 to 1,819,793 _RS08690
Group Condition AZOBR_RS08670 AZOBR_RS08675 AZOBR_RS08680 AZOBR_RS08685 AZOBR_RS08690
stress 2-pentyl-furan 1.780666667 mM -1.7 -0.1 N.D. +0.2 -0.5
nitrogen source L-Aspartic Acid (N) -2.2 +0.1 N.D. +0.1 +0.3
nitrogen source L-Asparagine (N) -1.7 +0.0 N.D. +0.1 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.6 -0.4 N.D. -0.1 -0.5
nitrogen source L-Aspartic Acid (N) -2.0 +0.2 N.D. +0.1 +0.1
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.5 -0.4 N.D. -0.1 -0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.8 -0.2 N.D. -0.1 -0.3
carbon source L-Glutamine (C) -0.6 -0.5 N.D. +0.0 -0.3
carbon source L-Proline (C) -1.3 -0.1 N.D. -0.1 +0.1
nitrogen source D-Alanine (N) -1.8 -0.1 N.D. +0.3 +0.5
nitrogen source L-Lysine (N) -1.2 -0.0 N.D. -0.1 +0.2
lb no stress control LB no stress control -1.3 +0.1 N.D. -0.1 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.4 -0.1 N.D. +0.3 -0.7
stress 2-pentyl-furan 1.335528 mM -1.2 +0.1 N.D. +0.0 +0.3
stress 3-octanone 6.3476 mM -1.0 +0.1 N.D. -0.1 +0.3
nitrogen source D-Alanine (N) -1.1 +0.2 N.D. +0.0 +0.2
stress Sisomicin 0.01 mg/ml -0.0 +0.3 N.D. +0.3 -1.0
stress 3-octanone 6.3476 mM -0.9 +0.4 N.D. +0.1 +0.2
stress 2-pentyl-furan 1.335528 mM -0.9 +0.2 N.D. +0.1 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 +0.0 N.D. -0.1 -0.3
stress Cobalt chloride 0.2 mM +0.3 -0.1 N.D. +0.7 -0.4
stress 2-Furfuraldehyde 0.2 vol% +0.6 +0.1 N.D. +0.5 -0.5
nitrogen source L-Isoleucine (N) +0.4 +0.5 N.D. +0.6 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.7 +0.4 N.D. -0.2 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.4 N.D. +0.5 +0.7
stress Paraquat dichloride 0.02 mg/ml +0.7 -0.0 N.D. +0.6 +0.2
motility inner cut, LB soft agar motility assay +0.5 +0.0 N.D. +0.8 +0.2
carbon source D-Gluconic Acid (C) +0.2 +0.4 N.D. +0.4 +0.6
stress Paraquat dichloride 0.04 mg/ml +1.0 -0.2 N.D. +0.5 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 +0.3 N.D. +0.6 +0.4
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