Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS08425 and AZOBR_RS08435 are separated by 1058 nucleotidesAZOBR_RS08435 and AZOBR_RS08440 are separated by 65 nucleotidesAZOBR_RS08440 and AZOBR_RS08445 are separated by 91 nucleotidesAZOBR_RS08445 and AZOBR_RS08450 are separated by 63 nucleotides AZOBR_RS08425: AZOBR_RS08425 - single-stranded DNA-binding protein, at 1,769,968 to 1,770,414 _RS08425 AZOBR_RS08435: AZOBR_RS08435 - transcriptional regulator, at 1,771,473 to 1,772,339 _RS08435 AZOBR_RS08440: AZOBR_RS08440 - kinase inhibitor, at 1,772,405 to 1,772,956 _RS08440 AZOBR_RS08445: AZOBR_RS08445 - delta-aminolevulinic acid dehydratase, at 1,773,048 to 1,774,061 _RS08445 AZOBR_RS08450: AZOBR_RS08450 - 3' 5'-cyclic-nucleotide phosphodiesterase, at 1,774,125 to 1,774,973 _RS08450
Group Condition AZOBR_RS08425 AZOBR_RS08435 AZOBR_RS08440 AZOBR_RS08445 AZOBR_RS08450
motility outer cut, LB soft agar motility assay N.D. -1.1 +0.4 N.D. -0.5
stress Bacitracin 0.25 mg/ml N.D. -0.4 -0.5 N.D. -0.1
stress Cobalt chloride 0.3 mM N.D. -0.8 +0.2 N.D. -0.2
nitrogen source D-Alanine (N) N.D. -0.9 +0.0 N.D. +0.1
stress 2-Furfuraldehyde 0.2 vol% N.D. -1.0 -0.0 N.D. +0.3
nitrogen source L-Serine (N) N.D. -0.7 +0.1 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.4 +0.5 N.D. +0.4
stress Sisomicin 0.01 mg/ml N.D. -0.7 +0.3 N.D. +0.1
stress 3-octanone 6.3476 mM N.D. +0.5 -0.5 N.D. -0.2
stress Cobalt chloride 0.2 mM N.D. +0.4 +0.1 N.D. -0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.7 +0.2 N.D. +0.4
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. -0.5 +0.5 N.D. +0.0
stress 3-octanone 6.3476 mM N.D. +0.5 -0.4 N.D. +0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.0 +0.7 N.D. -0.6
nitrogen source L-Histidine (N) N.D. +0.7 -0.4 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.4 -0.4 N.D. +0.2
stress D-Cycloserine 0.125 mg/ml N.D. +0.4 +0.6 N.D. -0.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.3 +0.5 N.D. +0.1
nitrogen source L-Glutamic (N) N.D. +0.4 -0.3 N.D. +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.4 +0.8 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.7 +0.2 N.D. -0.4
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -0.4 +0.5 N.D. +0.4
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM N.D. +0.7 -0.2 N.D. +0.1
lb LB N.D. +0.7 -0.2 N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.4 +0.5 N.D. -0.2
motility inner cut, LB soft agar motility assay N.D. +1.5 -0.6 N.D. -0.1
motility inner cut, LB soft agar motility assay N.D. -0.1 +1.3 N.D. -0.2
stress 1-octen-3-ol 0.7753276667 mM N.D. +0.8 +0.2 N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.9 +0.0 N.D. +0.1
stress 2-pentyl-furan 1.335528 mM N.D. +0.7 +0.2 N.D. +0.5
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