Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS08160 and AZOBR_RS08165 are separated by 351 nucleotidesAZOBR_RS08165 and AZOBR_RS08170 are separated by 24 nucleotidesAZOBR_RS08170 and AZOBR_RS08175 are separated by 354 nucleotidesAZOBR_RS08175 and AZOBR_RS08180 are separated by 97 nucleotides AZOBR_RS08160: AZOBR_RS08160 - sugar transporter, at 1,717,815 to 1,720,814 _RS08160 AZOBR_RS08165: AZOBR_RS08165 - hypothetical protein, at 1,721,166 to 1,721,663 _RS08165 AZOBR_RS08170: AZOBR_RS08170 - carbon-nitrogen family hydrolase, at 1,721,688 to 1,722,584 _RS08170 AZOBR_RS08175: AZOBR_RS08175 - transcriptional regulator, at 1,722,939 to 1,723,451 _RS08175 AZOBR_RS08180: AZOBR_RS08180 - hypothetical protein, at 1,723,549 to 1,724,859 _RS08180
Group Condition AZOBR_RS08160 AZOBR_RS08165 AZOBR_RS08170 AZOBR_RS08175 AZOBR_RS08180
stress 2-pentyl-furan 1.780666667 mM +0.3 N.D. -0.6 -0.9 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. -0.0 -1.0 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. -0.3 -0.4 -0.3
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM +0.0 N.D. +0.1 -1.3 -0.0
stress Benzethonium chloride 0.003 mM -0.0 N.D. +0.3 -1.1 -0.2
stress 2-pentyl-furan 1.335528 mM +0.0 N.D. +0.2 -1.6 +0.3
stress benzoic 0.29 mg/ml -0.2 N.D. -0.0 -1.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. +0.1 -0.8 -0.4
stress 3-octanone 5.2896 mM +0.0 N.D. -0.0 -1.1 +0.2
nitrogen source L-Glutamic (N) -0.3 N.D. +0.2 -1.0 +0.3
stress 2-pentyl-furan 1.780666667 mM +0.1 N.D. -0.3 -0.8 +0.2
stress 2-pentyl-furan 1.335528 mM +0.2 N.D. +0.3 -1.4 +0.4
carbon source a-Ketoglutaric (C) -0.2 N.D. -0.0 -0.6 +0.5
motility outer cut, LB soft agar motility assay +0.3 N.D. +0.1 -1.7 +1.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.2 N.D. +0.4 -0.6 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.2 N.D. +0.7 -0.1 -0.2
nitrogen source L-Isoleucine (N) +0.5 N.D. +0.5 +0.3 -0.6
motility inner cut, LB soft agar motility assay +0.1 N.D. -0.1 +0.9 -0.2
stress Paraquat dichloride 0.02 mg/ml -0.0 N.D. -0.1 +1.2 -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 N.D. +0.3 +0.6 -0.5
stress 3-octanone 6.3476 mM +0.2 N.D. -0.7 +1.3 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.2 N.D. -0.0 +1.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. -0.2 +0.9 +0.5
motility inner cut, LB soft agar motility assay +0.4 N.D. -0.2 +0.6 +0.4
carbon source L-Glutamine (C) +0.2 N.D. +0.2 +0.8 -0.1
stress 2-pentyl-furan 1.335528 mM +0.2 N.D. -0.0 +1.1 +0.0
motility inner cut, LB soft agar motility assay +0.3 N.D. +0.0 +1.0 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 N.D. +0.1 +0.5 +0.8
carbon source L-Malic (C) +0.3 N.D. +0.2 +0.9 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.2 N.D. +0.8 +0.9 +0.0
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