Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS08065 and AZOBR_RS08070 are separated by 26 nucleotidesAZOBR_RS08070 and AZOBR_RS08075 are separated by 18 nucleotidesAZOBR_RS08075 and AZOBR_RS08080 overlap by 4 nucleotidesAZOBR_RS08080 and AZOBR_RS08085 are separated by 49 nucleotides AZOBR_RS08065: AZOBR_RS08065 - urease subunit beta, at 1,701,614 to 1,701,919 _RS08065 AZOBR_RS08070: AZOBR_RS08070 - urease subunit alpha, at 1,701,946 to 1,703,655 _RS08070 AZOBR_RS08075: AZOBR_RS08075 - glutamate racemase, at 1,703,674 to 1,704,525 _RS08075 AZOBR_RS08080: AZOBR_RS08080 - urease accessory protein UreE, at 1,704,522 to 1,705,103 _RS08080 AZOBR_RS08085: AZOBR_RS08085 - urease accessory protein UreF, at 1,705,153 to 1,705,902 _RS08085
Group Condition AZOBR_RS08065 AZOBR_RS08070 AZOBR_RS08075 AZOBR_RS08080 AZOBR_RS08085
nitrogen source Urea (N) -6.9 -2.9 -0.5 -5.6 -6.8
nitrogen source Parabanic Acid (N) -4.6 -3.3 -0.5 -4.2 -5.6
nitrogen source Urea (N) -4.4 -2.9 -0.6 -4.9 -4.6
nitrogen source Parabanic Acid (N) -4.7 -3.1 -0.5 -4.5 -3.6
nitrogen source L-Citrulline (N) -1.9 -1.5 -0.4 -1.1 -1.9
nitrogen source L-Citrulline (N) -1.5 -1.7 +0.0 -1.3 -1.7
stress D-Cycloserine 0.125 mg/ml -1.6 -0.5 -5.1 +0.1 +1.2
nitrogen source L-Isoleucine (N) -1.5 +0.1 +0.2 -0.0 -1.4
carbon source D-Gluconic Acid (C) -0.9 -0.3 -0.0 +0.4 -1.8
nitrogen source L-Serine (N) -0.4 +0.1 -1.8 +0.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.5 +0.5 -1.1 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.2 -0.9 +0.2 +0.3 -2.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.9 +0.5 -0.0 -0.1
nitrogen source L-Serine (N) -0.1 +0.2 -1.7 +0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.9 +0.5 +0.4 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.3 +0.9 -0.3 -0.4
stress Benzethonium chloride 0.003 mM +0.7 +0.3 -0.0 -0.7 -0.6
stress 3-octanone 6.3476 mM +0.7 +0.5 -0.2 +0.3 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 -0.4 -0.0 +0.3 +1.1
stress 3-octanone 6.3476 mM +0.6 -0.5 -0.2 -0.3 +0.9
motility outer cut, LB soft agar motility assay +1.2 -0.3 +0.6 -0.9 +0.7
motility inner cut, LB soft agar motility assay +0.4 +0.6 +0.6 -0.6 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.9 +0.1 +0.8 -0.1 +0.2
motility inner cut, LB soft agar motility assay +0.7 +1.3 +0.4 -0.2 -0.2
stress Chlorite 1 mM +0.4 +0.8 +0.6 -0.3 +0.6
stress Sisomicin 0.01 mg/ml +1.4 -0.4 +0.8 +0.1 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.4 +0.2 +0.7 +0.1 +0.8
lb LB +0.3 +0.9 +0.4 +0.6 +0.4
motility inner cut, LB soft agar motility assay +0.5 +1.2 +0.4 +0.5 -0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.9 +1.0 +0.5 +0.1 +0.3
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