Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS07810 and AZOBR_RS07815 are separated by 233 nucleotidesAZOBR_RS07815 and AZOBR_RS07820 are separated by 399 nucleotidesAZOBR_RS07820 and AZOBR_RS07825 overlap by 4 nucleotidesAZOBR_RS07825 and AZOBR_RS07830 are separated by 301 nucleotides AZOBR_RS07810: AZOBR_RS07810 - poly(3-hydroxyalkanoate) synthetase, at 1,645,307 to 1,647,106 _RS07810 AZOBR_RS07815: AZOBR_RS07815 - hypothetical protein, at 1,647,340 to 1,647,696 _RS07815 AZOBR_RS07820: AZOBR_RS07820 - MarR family transcriptional regulator, at 1,648,096 to 1,648,548 _RS07820 AZOBR_RS07825: AZOBR_RS07825 - multidrug transporter CflA, at 1,648,545 to 1,649,744 _RS07825 AZOBR_RS07830: AZOBR_RS07830 - aminotransferase, at 1,650,046 to 1,651,320 _RS07830
Group Condition AZOBR_RS07810 AZOBR_RS07815 AZOBR_RS07820 AZOBR_RS07825 AZOBR_RS07830
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -3.6 N.D. -0.1 -0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -2.9 N.D. -0.1 +0.0 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -2.3 N.D. -0.0 -0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -2.3 N.D. -0.3 +0.0 N.D.
motility outer cut, LB soft agar motility assay -1.8 N.D. -0.3 -0.3 N.D.
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -2.7 N.D. +0.0 +0.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -2.7 N.D. +0.1 +0.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -2.1 N.D. -0.0 -0.2 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.9 N.D. -0.3 +0.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -2.2 N.D. -0.0 +0.2 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -2.3 N.D. -0.1 +0.4 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -1.7 N.D. -0.1 -0.1 N.D.
carbon source D-Fructose (C) -1.3 N.D. -0.1 -0.2 N.D.
nitrogen source L-Isoleucine (N) -0.8 N.D. -0.2 -0.3 N.D.
nitrogen source L-Glutamic (N) -1.1 N.D. +0.1 -0.3 N.D.
carbon source D-Fructose (C) -1.2 N.D. -0.1 +0.1 N.D.
motility inner cut, LB soft agar motility assay -0.5 N.D. -0.2 -0.5 N.D.
nitrogen source nitrate (N) -0.6 N.D. -0.6 +0.0 N.D.
nitrogen source Glucuronamide (N) -1.1 N.D. +0.1 -0.0 N.D.
stress Paraquat dichloride 0.04 mg/ml -0.6 N.D. -0.6 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 N.D. -0.9 -0.3 N.D.
nitrogen source L-Lysine (N) -0.8 N.D. +0.3 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 N.D. -0.6 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 N.D. -0.7 +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 N.D. -0.7 +0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 N.D. -0.5 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 N.D. -0.2 +0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.1 N.D. -0.2 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 N.D. +0.4 +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.1 N.D. +0.1 +0.3 N.D.
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