Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS07315 and AZOBR_RS07320 are separated by 200 nucleotidesAZOBR_RS07320 and AZOBR_RS07330 are separated by 1101 nucleotidesAZOBR_RS07330 and AZOBR_RS07335 are separated by 3 nucleotidesAZOBR_RS07335 and AZOBR_RS07340 are separated by 4 nucleotides AZOBR_RS07315: AZOBR_RS07315 - putative plasmid stabilization system protein, at 1,543,543 to 1,543,818 _RS07315 AZOBR_RS07320: AZOBR_RS07320 - 30S ribosomal protein S2, at 1,544,019 to 1,544,804 _RS07320 AZOBR_RS07330: AZOBR_RS07330 - uridylate kinase, at 1,545,906 to 1,546,661 _RS07330 AZOBR_RS07335: AZOBR_RS07335 - ribosome recycling factor, at 1,546,665 to 1,547,222 _RS07335 AZOBR_RS07340: AZOBR_RS07340 - UDP pyrophosphate synthase, at 1,547,227 to 1,547,964 _RS07340
Group Condition AZOBR_RS07315 AZOBR_RS07320 AZOBR_RS07330 AZOBR_RS07335 AZOBR_RS07340
stress 2-pentyl-furan 2.671 mM -0.5 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. N.D. N.D. N.D.
stress Cobalt chloride 0.2 mM -0.5 N.D. N.D. N.D. N.D.
nitrogen source L-Isoleucine (N) -0.5 N.D. N.D. N.D. N.D.
stress 1-octen-3-ol 0.6030326667 mM -0.4 N.D. N.D. N.D. N.D.
stress 2-pentyl-furan 1.780666667 mM -0.4 N.D. N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay -0.4 N.D. N.D. N.D. N.D.
lb no stress control LB no stress control -0.4 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.4 N.D. N.D. N.D. N.D.
stress 1-octen-3-ol 0.6784116667 mM -0.3 N.D. N.D. N.D. N.D.
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -0.3 N.D. N.D. N.D. N.D.
stress 2-pentyl-furan 1.780666667 mM -0.3 N.D. N.D. N.D. N.D.
stress 1-octen-3-ol 0.7753276667 mM -0.3 N.D. N.D. N.D. N.D.
stress 2-pentyl-furan 1.335528 mM -0.3 N.D. N.D. N.D. N.D.
lb LB -0.3 N.D. N.D. N.D. N.D.
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -0.3 N.D. N.D. N.D. N.D.
stress 3-octanone 4.2317 mM -0.3 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.3 N.D. N.D. N.D. N.D.
nitrogen source Cytosine (N) +0.3 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.3 N.D. N.D. N.D. N.D.
carbon source D-Fructose (C) +0.3 N.D. N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.3 N.D. N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.3 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 N.D. N.D. N.D. N.D.
anaerobic; rich media LB; anaerobic +0.3 N.D. N.D. N.D. N.D.
stress 2-pentyl-furan 1.335528 mM +0.3 N.D. N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.4 N.D. N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.5 N.D. N.D. N.D. N.D.
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