Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS06555 and AZOBR_RS06560 are separated by 304 nucleotidesAZOBR_RS06560 and AZOBR_RS06565 are separated by 50 nucleotidesAZOBR_RS06565 and AZOBR_RS06570 are separated by 175 nucleotidesAZOBR_RS06570 and AZOBR_RS06575 are separated by 176 nucleotides AZOBR_RS06555: AZOBR_RS06555 - Branched-chain-amino-acid transaminase (EC 2.6.1.42) (from data), at 1,387,614 to 1,388,828 _RS06555 AZOBR_RS06560: AZOBR_RS06560 - acetolactate synthase, at 1,389,133 to 1,390,896 _RS06560 AZOBR_RS06565: AZOBR_RS06565 - acetolactate synthase, at 1,390,947 to 1,391,453 _RS06565 AZOBR_RS06570: AZOBR_RS06570 - ketol-acid reductoisomerase, at 1,391,629 to 1,392,648 _RS06570 AZOBR_RS06575: AZOBR_RS06575 - PhoU family transcriptional regulator, at 1,392,825 to 1,393,529 _RS06575
Group Condition AZOBR_RS06555 AZOBR_RS06560 AZOBR_RS06565 AZOBR_RS06570 AZOBR_RS06575
carbon source L-Leucine (C) -1.9 -1.2 -6.6 N.D. -0.2
carbon source L-Proline (C) -0.2 -2.5 -4.8 N.D. -0.3
carbon source L-Proline (C) -0.6 -1.2 -5.1 N.D. -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.4 -1.7 -5.1 N.D. -0.3
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.6 -4.0 -1.5 N.D. -0.2
stress 2-pentyl-furan 1.335528 mM -0.3 -2.7 -2.5 N.D. +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.2 -3.9 -1.0 N.D. -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.1 -3.9 -1.3 N.D. +0.1
stress 2-pentyl-furan 1.780666667 mM -0.3 -2.6 -2.4 N.D. +0.2
carbon source L-Glutamine (C) -0.1 -2.0 -2.6 N.D. -0.4
stress 2-pentyl-furan 2.671 mM -0.6 -1.6 -2.4 N.D. -0.3
stress 2-pentyl-furan 1.780666667 mM +0.0 -2.6 -2.4 N.D. +0.2
nitrogen source L-Leucine (N) -1.6 -1.9 -1.3 N.D. +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.0 -4.0 -0.8 N.D. +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.0 -3.4 -1.0 N.D. -0.3
stress 2-pentyl-furan 1.335528 mM +0.1 -2.5 -2.4 N.D. +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.1 -3.3 -0.9 N.D. -0.2
stress 1-octen-3-ol 0.6784116667 mM -0.0 -2.2 -2.4 N.D. +0.0
stress 1-octen-3-ol 0.6030326667 mM -0.3 -1.8 -2.4 N.D. -0.0
carbon source D-Fructose (C) -0.3 -2.6 -1.5 N.D. -0.1
anaerobic; rich media LB; anaerobic -0.1 -4.3 -0.3 N.D. +0.3
stress 1-octen-3-ol 0.6784116667 mM -0.3 -1.8 -2.4 N.D. +0.1
stress 2-pentyl-furan 1.780666667 mM +0.0 -2.3 -2.4 N.D. +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.0 -4.0 -0.5 N.D. +0.2
stress 3-octanone 5.2896 mM -0.4 -1.5 -2.5 N.D. +0.3
stress 2-pentyl-furan 1.335528 mM +0.4 -2.2 -2.3 N.D. +0.2
stress 3-octanone 5.2896 mM -0.2 -1.6 -2.3 N.D. +0.3
stress 3-octanone 6.3476 mM +0.1 -1.5 -2.4 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 +0.1 -3.7 N.D. +1.0
motility inner cut, LB soft agar motility assay +0.1 -3.3 +0.9 N.D. +0.7
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