Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS06265 and AZOBR_RS06270 are separated by 180 nucleotidesAZOBR_RS06270 and AZOBR_RS06275 overlap by 4 nucleotidesAZOBR_RS06275 and AZOBR_RS06280 are separated by 27 nucleotidesAZOBR_RS06280 and AZOBR_RS06285 overlap by 54 nucleotides AZOBR_RS06265: AZOBR_RS06265 - methyl-accepting chemotaxis protein, at 1,325,492 to 1,327,894 _RS06265 AZOBR_RS06270: AZOBR_RS06270 - ligase, at 1,328,075 to 1,329,259 _RS06270 AZOBR_RS06275: AZOBR_RS06275 - heptosyltransferase, at 1,329,256 to 1,330,200 _RS06275 AZOBR_RS06280: AZOBR_RS06280 - membrane protein of unknown function, at 1,330,228 to 1,331,316 _RS06280 AZOBR_RS06285: AZOBR_RS06285 - epoxyqueuosine reductase, at 1,331,263 to 1,332,279 _RS06285
Group Condition AZOBR_RS06265 AZOBR_RS06270 AZOBR_RS06275 AZOBR_RS06280 AZOBR_RS06285
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. -1.0 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -1.7 +0.5
nitrogen source L-Glutamic (N) N.D. N.D. N.D. -1.0 -0.2
stress 2-Furfuraldehyde 0.2 vol% N.D. N.D. N.D. -1.0 -0.2
nitrogen source L-Leucine (N) N.D. N.D. N.D. -0.3 -0.9
stress Paraquat dichloride 0.04 mg/ml N.D. N.D. N.D. -0.9 -0.2
nitrogen source L-Threonine (N) N.D. N.D. N.D. +0.2 -1.2
nitrogen source L-Phenylalanine (N) N.D. N.D. N.D. -0.6 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -0.9 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -1.3 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -1.3 +0.6
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. N.D. -1.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -1.1 +0.4
stress 1-octen-3-ol 0.6784116667 mM N.D. N.D. N.D. -0.8 +0.1
nitrogen source L-Leucine (N) N.D. N.D. N.D. +0.4 -1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -1.6 +1.0
nitrogen source Cytosine (N) N.D. N.D. N.D. +0.2 -0.7
lb LB N.D. N.D. N.D. -0.7 +0.3
nitrogen source L-Isoleucine (N) N.D. N.D. N.D. +0.6 -0.9
carbon source D-Gluconic Acid (C) N.D. N.D. N.D. +0.6 -0.8
carbon source L-Leucine (C) N.D. N.D. N.D. +0.5 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -0.6 +0.5
stress D-Cycloserine 0.125 mg/ml N.D. N.D. N.D. +0.7 -0.4
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. N.D. N.D. +0.9 -0.4
nitrogen source L-Phenylalanine (N) N.D. N.D. N.D. +0.7 -0.2
stress nitrate 100 mM N.D. N.D. N.D. +1.0 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. +1.2 -0.4
lb LB N.D. N.D. N.D. +0.5 +0.3
stress Nickel (II) chloride 0.5 mM N.D. N.D. N.D. +0.8 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.8 +0.5
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