Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS06180 and AZOBR_RS06185 are separated by 243 nucleotidesAZOBR_RS06185 and AZOBR_RS06190 are separated by 57 nucleotidesAZOBR_RS06190 and AZOBR_RS06195 are separated by 156 nucleotidesAZOBR_RS06195 and AZOBR_RS06200 are separated by 320 nucleotides AZOBR_RS06180: AZOBR_RS06180 - flgE (modular protein), at 1,309,382 to 1,310,752 _RS06180 AZOBR_RS06185: AZOBR_RS06185 - carbonic anhydrase, at 1,310,996 to 1,311,688 _RS06185 AZOBR_RS06190: AZOBR_RS06190 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 1,311,746 to 1,312,339 _RS06190 AZOBR_RS06195: AZOBR_RS06195 - hypothetical protein, at 1,312,496 to 1,313,113 _RS06195 AZOBR_RS06200: AZOBR_RS06200 - NAD(P)H quinone oxidoreductase, at 1,313,434 to 1,314,441 _RS06200
Group Condition AZOBR_RS06180 AZOBR_RS06185 AZOBR_RS06190 AZOBR_RS06195 AZOBR_RS06200
motility outer cut, LB soft agar motility assay +0.5 N.D. N.D. N.D. -1.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -1.2 N.D. N.D. N.D. +0.2
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -0.3 N.D. N.D. N.D. -0.6
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -0.3 N.D. N.D. N.D. -0.5
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM -0.3 N.D. N.D. N.D. -0.5
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -0.3 N.D. N.D. N.D. -0.5
carbon source D,L-Lactate (C) -0.3 N.D. N.D. N.D. -0.4
carbon source L-Lactate (C) -0.1 N.D. N.D. N.D. -0.6
motility inner cut, LB soft agar motility assay -0.7 N.D. N.D. N.D. +0.0
nitrogen source D-Alanine (N) -0.4 N.D. N.D. N.D. -0.2
stress 3-octanone 5.2896 mM -0.4 N.D. N.D. N.D. -0.2
lb LB +0.2 N.D. N.D. N.D. -0.5
motility inner cut, LB soft agar motility assay +0.2 N.D. N.D. N.D. -0.4
nitrogen source casaminos (N) -0.4 N.D. N.D. N.D. +0.2
carbon source D,L-Lactate (C) -0.3 N.D. N.D. N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. N.D. N.D. +0.4
carbon source pyruvate (C) +0.4 N.D. N.D. N.D. -0.3
stress Benzethonium chloride 0.003 mM +0.6 N.D. N.D. N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 N.D. N.D. N.D. +0.7
nitrogen source L-Glutamic (N) -0.2 N.D. N.D. N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. N.D. N.D. +0.6
stress 1-octen-3-ol 0.6030326667 mM +0.2 N.D. N.D. N.D. +0.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.0 N.D. N.D. N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 N.D. N.D. N.D. +0.1
stress Bacitracin 0.25 mg/ml +0.3 N.D. N.D. N.D. +0.3
nitrogen source Glucuronamide (N) +0.1 N.D. N.D. N.D. +0.5
nitrogen source D-Alanine (N) +0.4 N.D. N.D. N.D. +0.2
nitrogen source L-Histidine (N) +0.4 N.D. N.D. N.D. +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.5 N.D. N.D. N.D. +0.2
motility inner cut, LB soft agar motility assay +0.6 N.D. N.D. N.D. +0.6
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