Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS06145 and AZOBR_RS06150 are separated by 120 nucleotidesAZOBR_RS06150 and AZOBR_RS06155 are separated by 202 nucleotidesAZOBR_RS06155 and AZOBR_RS06160 are separated by 43 nucleotidesAZOBR_RS06160 and AZOBR_RS06165 are separated by 13 nucleotides AZOBR_RS06145: AZOBR_RS06145 - N5-carboxyaminoimidazole ribonucleotide mutase, at 1,303,788 to 1,304,297 _RS06145 AZOBR_RS06150: AZOBR_RS06150 - small protein containing a coiled-coil domain containing protein, at 1,304,418 to 1,304,618 _RS06150 AZOBR_RS06155: AZOBR_RS06155 - 6-phosphofructokinase, at 1,304,821 to 1,305,906 _RS06155 AZOBR_RS06160: AZOBR_RS06160 - hypothetical protein, at 1,305,950 to 1,306,408 _RS06160 AZOBR_RS06165: AZOBR_RS06165 - GCN5 family N-acetyltransferase, at 1,306,422 to 1,307,009 _RS06165
Group Condition AZOBR_RS06145 AZOBR_RS06150 AZOBR_RS06155 AZOBR_RS06160 AZOBR_RS06165
motility outer cut, LB soft agar motility assay N.D. -3.5 -0.6 +0.4 -0.9
stress 2-pentyl-furan 2.671 mM N.D. -1.2 -0.6 -2.5 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 +0.1 -4.9 +0.0
stress Paraquat dichloride 0.04 mg/ml N.D. -0.5 -0.4 -2.1 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.7 +0.2 -1.6 -0.3
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM N.D. -1.5 -0.2 -0.9 +0.1
stress 3-octanone 4.2317 mM N.D. -1.5 -0.1 -0.6 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.4 -1.4 -0.1 -0.3
stress 1-octen-3-ol 0.6784116667 mM N.D. -1.4 -0.3 -0.5 -0.0
stress 1-octen-3-ol 0.7753276667 mM N.D. -1.4 -0.2 -0.5 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.5 +0.2 -2.0 +0.5
carbon source D-Gluconic Acid (C) N.D. -1.0 -0.4 +0.3 -0.7
stress 3-octanone 6.3476 mM N.D. -1.0 -0.8 -0.2 +0.2
stress 3-octanone 5.2896 mM N.D. -1.4 +0.2 -0.3 -0.2
stress 2-pentyl-furan 1.780666667 mM N.D. -1.3 +0.2 -0.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.0 +1.1 -2.0 +0.3
stress 1-octen-3-ol 0.6030326667 mM N.D. -1.5 +0.5 -0.6 +0.1
carbon source Ethanol (C) N.D. -0.8 -0.5 +0.3 -0.5
stress 2-pentyl-furan 1.780666667 mM N.D. -1.3 +0.1 -0.6 +0.2
stress 3-octanone 6.3476 mM N.D. -0.3 +0.4 -1.1 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.6 -0.9 -1.3 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.3 +0.5 -1.6 +0.1
stress 2-Furfuraldehyde 0.2 vol% N.D. -1.0 -0.6 -0.3 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.2 +0.5 +0.3 -0.6
motility inner cut, LB soft agar motility assay N.D. +0.3 +0.5 -1.8 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.1 +1.5 -1.0 +0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.7 -0.8 +0.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.9 +0.4 -0.7 +0.3
stress Sisomicin 0.01 mg/ml N.D. -1.1 +0.5 +1.2 +0.3
nitrogen source L-Isoleucine (N) N.D. +1.9 -0.6 -0.3 -0.1
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