Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS05495 and AZOBR_RS05500 are separated by 219 nucleotidesAZOBR_RS05500 and AZOBR_RS05505 are separated by 3 nucleotidesAZOBR_RS05505 and AZOBR_RS05510 are separated by 46 nucleotidesAZOBR_RS05510 and AZOBR_RS05515 are separated by 305 nucleotides AZOBR_RS05495: AZOBR_RS05495 - homocitrate synthase, at 1,169,448 to 1,170,593 _RS05495 AZOBR_RS05500: AZOBR_RS05500 - cysteine desulfurase, at 1,170,813 to 1,172,018 _RS05500 AZOBR_RS05505: AZOBR_RS05505 - nitrogen fixation protein NifU, at 1,172,022 to 1,172,939 _RS05505 AZOBR_RS05510: AZOBR_RS05510 - hypothetical protein, at 1,172,986 to 1,173,366 _RS05510 AZOBR_RS05515: AZOBR_RS05515 - hypothetical protein, at 1,173,672 to 1,174,145 _RS05515
Group Condition AZOBR_RS05495 AZOBR_RS05500 AZOBR_RS05505 AZOBR_RS05510 AZOBR_RS05515
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -4.8 -2.3 -2.7 N.D. -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -4.0 -2.2 -2.1 N.D. +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -3.5 -1.9 -2.2 N.D. +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -2.5 -2.4 -2.0 N.D. -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -2.9 -1.8 -1.9 N.D. -0.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -1.4 -1.3 -1.1 N.D. +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.9 -0.6 -0.4 N.D. +0.8
stress 2-pentyl-furan 1.335528 mM -1.8 -0.0 +0.1 N.D. -0.1
stress 2-pentyl-furan 2.671 mM -1.6 -0.1 -0.1 N.D. -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.4 -0.3 N.D. -1.1
stress 2-pentyl-furan 1.335528 mM -0.6 -0.1 -0.3 N.D. -0.7
stress 2-Furfuraldehyde 0.2 vol% -0.3 +0.2 -0.3 N.D. -1.2
stress 2-pentyl-furan 1.780666667 mM -0.8 -0.5 -0.2 N.D. -0.0
stress Vancomycin 0.03 mg/ml -0.2 -0.1 +0.0 N.D. -1.1
stress Cobalt chloride 0.2 mM -0.5 -0.3 +0.0 N.D. -0.6
nitrogen source casaminos (N) -0.6 +0.3 -0.0 N.D. -1.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.6 -0.4 -0.2 N.D. +0.3
carbon source D-Gluconic Acid (C) -0.4 +0.3 -0.1 N.D. -0.6
lb LB +0.3 -0.2 +0.0 N.D. -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.8 -0.2 -0.2 N.D. +0.7
carbon source L-Glutamine (C) +0.4 +0.0 +0.1 N.D. -1.0
stress 3-octanone 6.3476 mM -0.4 -0.2 -0.2 N.D. +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.5 -0.1 -0.0 N.D. +1.1
stress 2-pentyl-furan 1.780666667 mM +0.6 -0.3 +0.2 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.1 +0.1 N.D. +1.1
dmso control DMSO control +0.8 -0.1 +0.1 N.D. +0.4
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM +0.5 +0.1 +0.0 N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.1 -0.1 N.D. +1.0
stress Sisomicin 0.01 mg/ml +0.4 -0.0 +0.2 N.D. +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +0.5 +0.2 N.D. +0.4
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