Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS05270 and AZOBR_RS05275 are separated by 213 nucleotidesAZOBR_RS05275 and AZOBR_RS05280 are separated by 216 nucleotidesAZOBR_RS05280 and AZOBR_RS05285 are separated by 19 nucleotidesAZOBR_RS05285 and AZOBR_RS05290 are separated by 207 nucleotides AZOBR_RS05270: AZOBR_RS05270 - exported protein of unknown function, at 1,119,743 to 1,120,132 _RS05270 AZOBR_RS05275: AZOBR_RS05275 - hypothetical protein, at 1,120,346 to 1,121,203 _RS05275 AZOBR_RS05280: AZOBR_RS05280 - fuculose phosphate aldolase, at 1,121,420 to 1,122,118 _RS05280 AZOBR_RS05285: AZOBR_RS05285 - histidine kinase, at 1,122,138 to 1,124,213 _RS05285 AZOBR_RS05290: AZOBR_RS05290 - extradiol ring-cleavage dioxygenase, at 1,124,421 to 1,125,197 _RS05290
Group Condition AZOBR_RS05270 AZOBR_RS05275 AZOBR_RS05280 AZOBR_RS05285 AZOBR_RS05290
motility outer cut, LB soft agar motility assay -1.0 -1.5 +0.2 -0.0 +0.1
motility inner cut, LB soft agar motility assay -0.7 -0.8 -0.2 -0.5 +0.0
nitrogen source Cytosine (N) -0.6 -0.3 -0.4 -0.0 -0.8
stress 2-Furfuraldehyde 0.2 vol% -0.8 -0.9 -0.2 -0.2 +0.1
stress 2-pentyl-furan 1.780666667 mM -0.7 -0.3 -0.3 -0.3 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.8 -0.8 +0.0 -0.0 -0.2
lb LB +0.3 -0.4 -0.3 -0.1 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.4 -0.3 -0.0 -0.3
stress Cobalt chloride 0.2 mM -0.3 -0.0 -0.2 -0.3 -0.9
nitrogen source L-Threonine (N) +0.1 -1.4 -0.1 +0.0 +0.1
nitrogen source casaminos (N) +0.2 -0.6 +0.0 +0.5 -0.6
motility inner cut, LB soft agar motility assay +0.6 -0.6 -0.2 +0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.8 +0.2 +0.8 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +0.6 -0.4 +0.1 -0.7
stress Paraquat dichloride 0.02 mg/ml +0.5 -0.8 +0.3 +0.1 +0.0
nitrogen source L-Glutamic (N) +0.9 +0.7 -1.0 -0.1 -0.2
stress Benzethonium chloride 0.003 mM +0.5 +0.1 +0.0 -0.8 +0.5
carbon source D-Gluconic Acid (C) +0.3 +0.3 -0.9 -0.2 +0.8
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.5 +0.1 +0.2 +0.3 -0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.6 -0.3 +0.4 +0.3 -0.4
stress D-Cycloserine 0.125 mg/ml +0.9 +0.1 -0.6 -0.2 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.1 -0.5 +0.3 +0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.5 -0.1 +0.7 +0.3 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.7 -0.0 +0.1 +0.6 -0.5
nitrogen source L-Leucine (N) +0.2 -0.4 +0.3 +0.2 +0.6
lb LB +0.9 -0.2 +0.2 -0.2 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.9 +0.1 +0.2 +0.5 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.6 -0.1 +0.3 +0.6 -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.9 +0.2 +0.7 -0.1 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.8 -0.2 +0.3 +0.6
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