Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS05165 and AZOBR_RS05170 are separated by 153 nucleotidesAZOBR_RS05170 and AZOBR_RS05175 are separated by 79 nucleotidesAZOBR_RS05175 and AZOBR_RS05180 are separated by 8 nucleotidesAZOBR_RS05180 and AZOBR_RS05185 are separated by 455 nucleotides AZOBR_RS05165: AZOBR_RS05165 - carnitine dehydratase, at 1,099,412 to 1,100,524 _RS05165 AZOBR_RS05170: AZOBR_RS05170 - hypothetical protein, at 1,100,678 to 1,100,869 _RS05170 AZOBR_RS05175: AZOBR_RS05175 - ATP-dependent Clp protease ATP-binding protein, at 1,100,949 to 1,103,264 _RS05175 AZOBR_RS05180: AZOBR_RS05180 - Clp protease ClpS, at 1,103,273 to 1,103,599 _RS05180 AZOBR_RS05185: AZOBR_RS05185 - hypothetical protein, at 1,104,055 to 1,104,540 _RS05185
Group Condition AZOBR_RS05165 AZOBR_RS05170 AZOBR_RS05175 AZOBR_RS05180 AZOBR_RS05185
carbon source D-Gluconic Acid (C) +0.0 -0.6 -2.0 -4.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -3.7 +0.1 -2.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.1 -0.0 -2.2 -2.8 N.D.
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.1 -0.5 -2.7 -1.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -1.2 -0.6 -2.7 N.D.
nitrogen source L-Threonine (N) +0.1 +0.3 -0.2 -4.7 N.D.
carbon source a-Ketoglutaric (C) -0.2 -0.1 -2.8 -1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 +0.1 -0.0 -4.4 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.1 +0.0 -3.0 -1.4 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.1 -0.2 -2.8 -1.3 N.D.
motility inner cut, LB soft agar motility assay -0.4 -3.9 -0.5 +0.6 N.D.
carbon source a-Ketoglutaric (C) +0.0 -0.3 -2.7 -0.9 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 -0.3 -2.0 -1.6 N.D.
stress 2-Furfuraldehyde 0.2 vol% -0.2 -0.6 -2.0 -0.8 N.D.
nitrogen source L-Phenylalanine (N) -0.3 +0.2 -1.5 -1.9 N.D.
nitrogen source L-Glutamic (N) -0.2 -0.9 -1.6 -0.8 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.2 +0.3 -2.2 -1.7 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.3 +0.1 -2.6 -1.0 N.D.
stress Chlorite 1 mM -0.1 -0.6 -0.2 -2.3 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.1 -0.3 -2.4 -0.5 N.D.
nitrogen source L-Threonine (N) +0.1 +0.1 -1.9 -1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -3.7 +0.8 -0.3 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.3 +0.1 -1.6 -1.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.4 -0.2 -2.7 N.D.
lb LB -0.0 +0.1 +0.3 -2.7 N.D.
nitrogen source Cytosine (N) -0.1 +0.4 -1.7 -0.9 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 -0.1 +0.7 -2.2 N.D.
nitrogen source L-Histidine (N) +0.2 +0.8 -0.9 -1.8 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.3 -2.8 +0.1 +0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -1.8 +0.0 +1.1 N.D.
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