Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS05125 and AZOBR_RS05130 are separated by 128 nucleotidesAZOBR_RS05130 and AZOBR_RS05135 are separated by 198 nucleotidesAZOBR_RS05135 and AZOBR_RS05140 are separated by 139 nucleotidesAZOBR_RS05140 and AZOBR_RS05145 are separated by 408 nucleotides AZOBR_RS05125: AZOBR_RS05125 - ribonucleotide-diphosphate reductase subunit alpha, at 1,087,087 to 1,090,806 _RS05125 AZOBR_RS05130: AZOBR_RS05130 - hypothetical protein, at 1,090,935 to 1,091,417 _RS05130 AZOBR_RS05135: AZOBR_RS05135 - septum formation initiator, at 1,091,616 to 1,093,247 _RS05135 AZOBR_RS05140: AZOBR_RS05140 - flagellin, at 1,093,387 to 1,095,252 _RS05140 AZOBR_RS05145: AZOBR_RS05145 - endoribonuclease, at 1,095,661 to 1,096,128 _RS05145
Group Condition AZOBR_RS05125 AZOBR_RS05130 AZOBR_RS05135 AZOBR_RS05140 AZOBR_RS05145
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 -0.3 -1.6 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 -0.1 -0.8 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.0 +0.1 -1.3 -1.1
stress Chlorite 1 mM N.D. -0.1 -0.0 -1.5 -0.6
nitrogen source L-Threonine (N) N.D. +0.2 +0.1 +0.4 -2.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 +0.2 -0.2 -1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.0 -0.1 -0.9 -0.6
nitrogen source L-Threonine (N) N.D. +0.4 +0.3 +0.2 -2.5
carbon source L-Leucine (C) N.D. -0.1 -0.3 -0.2 -1.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -0.0 -0.2 -1.1 -0.3
motility outer cut, LB soft agar motility assay N.D. -0.3 -0.1 -1.3 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.0 +0.1 -1.5 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.5 -0.0 -1.9 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.5 +0.1 -0.6 -1.1
stress Paraquat dichloride 0.04 mg/ml N.D. -0.3 -0.2 +0.2 -0.6
nitrogen source L-Isoleucine (N) N.D. -0.1 -0.3 +0.6 -1.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.2 +0.1 -1.2 +0.2
stress Benzethonium chloride 0.003 mM N.D. +0.3 +0.1 -0.8 -0.2
carbon source L-Malic (C) N.D. -0.3 -0.1 -0.5 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.5 +0.4 -0.1 -1.2
nitrogen source L-Leucine (N) N.D. -0.2 -0.0 +0.6 -0.8
motility inner cut, LB soft agar motility assay N.D. -0.3 +0.0 -0.7 +0.7
stress Cobalt chloride 0.3 mM N.D. +0.3 +0.3 -0.7 +0.1
motility inner cut, LB soft agar motility assay N.D. -0.1 -0.4 -0.3 +0.9
stress 2-pentyl-furan 2.671 mM N.D. -0.3 +0.0 +0.0 +1.0
stress D-Cycloserine 0.125 mg/ml N.D. +0.2 +0.5 -0.3 +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.8 +0.4 -0.7 +0.4
nitrogen source L-Glutamic (N) N.D. -0.2 +0.4 +0.5 +0.3
stress Sisomicin 0.01 mg/ml N.D. +0.4 +0.2 +0.2 +0.7
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. +0.7 +0.4 -0.1 +0.8
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