Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS05115 and AZOBR_RS05120 are separated by 95 nucleotidesAZOBR_RS05120 and AZOBR_RS05125 are separated by 542 nucleotidesAZOBR_RS05125 and AZOBR_RS05130 are separated by 128 nucleotidesAZOBR_RS05130 and AZOBR_RS05135 are separated by 198 nucleotides AZOBR_RS05115: AZOBR_RS05115 - hypothetical protein, at 1,085,586 to 1,085,957 _RS05115 AZOBR_RS05120: AZOBR_RS05120 - hypothetical protein, at 1,086,053 to 1,086,544 _RS05120 AZOBR_RS05125: AZOBR_RS05125 - ribonucleotide-diphosphate reductase subunit alpha, at 1,087,087 to 1,090,806 _RS05125 AZOBR_RS05130: AZOBR_RS05130 - hypothetical protein, at 1,090,935 to 1,091,417 _RS05130 AZOBR_RS05135: AZOBR_RS05135 - septum formation initiator, at 1,091,616 to 1,093,247 _RS05135
Group Condition AZOBR_RS05115 AZOBR_RS05120 AZOBR_RS05125 AZOBR_RS05130 AZOBR_RS05135
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -0.3 -0.7
stress Cobalt chloride 0.2 mM N.D. N.D. N.D. -0.4 -0.3
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. -0.1 -0.4
stress Paraquat dichloride 0.04 mg/ml N.D. N.D. N.D. -0.3 -0.2
carbon source L-Malic (C) N.D. N.D. N.D. -0.3 -0.1
carbon source L-Leucine (C) N.D. N.D. N.D. -0.1 -0.3
carbon source Glycerol (C) N.D. N.D. N.D. -0.4 +0.0
stress 3-octanone 6.3476 mM N.D. N.D. N.D. -0.4 +0.2
carbon source a-Ketoglutaric (C) N.D. N.D. N.D. -0.3 +0.2
nitrogen source L-Glutamic (N) N.D. N.D. N.D. -0.2 +0.4
stress 3-octanone 6.3476 mM N.D. N.D. N.D. -0.1 +0.4
nitrogen source casaminos (N) N.D. N.D. N.D. +0.3 +0.2
nitrogen source Parabanic Acid (N) N.D. N.D. N.D. +0.2 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. N.D. N.D. +0.5 -0.0
carbon source pyruvate (C) N.D. N.D. N.D. +0.5 +0.0
stress Sisomicin 0.01 mg/ml N.D. N.D. N.D. +0.4 +0.2
carbon source Glycerol (C) N.D. N.D. N.D. +0.4 +0.2
stress Cobalt chloride 0.3 mM N.D. N.D. N.D. +0.3 +0.3
carbon source L-Glutamine (C) N.D. N.D. N.D. +0.6 +0.1
nitrogen source L-Threonine (N) N.D. N.D. N.D. +0.4 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. +0.5 +0.1
nitrogen source D-Alanine (N) N.D. N.D. N.D. +0.6 +0.1
nitrogen source Ammonium chloride (N) N.D. N.D. N.D. +0.4 +0.3
carbon source succinate (C) N.D. N.D. N.D. +0.5 +0.2
nitrogen source L-Aspartic Acid (N) N.D. N.D. N.D. +0.3 +0.4
nitrogen source Glucuronamide (N) N.D. N.D. N.D. +0.4 +0.3
stress D-Cycloserine 0.125 mg/ml N.D. N.D. N.D. +0.2 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. +0.5 +0.4
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. N.D. N.D. +0.7 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. N.D. N.D. +0.8 +0.4
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