Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS05010 and AZOBR_RS05020 are separated by 759 nucleotidesAZOBR_RS05020 and AZOBR_RS05025 are separated by 896 nucleotidesAZOBR_RS05025 and AZOBR_RS05030 are separated by 76 nucleotidesAZOBR_RS05030 and AZOBR_RS05035 are separated by 332 nucleotides AZOBR_RS05010: AZOBR_RS05010 - transposase, at 1,061,394 to 1,061,765 _RS05010 AZOBR_RS05020: AZOBR_RS05020 - hypothetical protein, at 1,062,525 to 1,062,797 _RS05020 AZOBR_RS05025: AZOBR_RS05025 - DNA mismatch repair protein MutL, at 1,063,694 to 1,065,592 _RS05025 AZOBR_RS05030: AZOBR_RS05030 - hypothetical protein, at 1,065,669 to 1,066,511 _RS05030 AZOBR_RS05035: AZOBR_RS05035 - hypothetical protein, at 1,066,844 to 1,068,049 _RS05035
Group Condition AZOBR_RS05010 AZOBR_RS05020 AZOBR_RS05025 AZOBR_RS05030 AZOBR_RS05035
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.4 -0.7 +0.1 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.9 -0.2 -0.4 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.3 -0.0 -0.1 -0.0
motility inner cut, LB soft agar motility assay N.D. -0.4 -0.2 -0.6 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.0 -0.4 -0.7 -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.5 -0.1 -0.6 -0.1
lb LB N.D. -0.6 -0.2 -0.3 +0.1
stress Cobalt chloride 0.3 mM N.D. -0.7 +0.1 -0.3 -0.1
carbon source L-Lactate (C) N.D. -0.7 -0.1 -0.2 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.6 +0.1 -0.4 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.9 -0.1 +0.0 +0.1
carbon source D-Gluconic Acid (C) N.D. -1.4 +0.3 +0.0 +0.3
nitrogen source L-Aspartic Acid (N) N.D. -0.8 +0.1 +0.1 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 +0.1 -0.5 +0.4
nitrogen source L-Threonine (N) N.D. -0.9 +0.2 +0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.6 +0.1 +0.3 +0.2
nitrogen source L-Phenylalanine (N) N.D. -0.5 -0.1 +0.4 +0.1
motility outer cut, LB soft agar motility assay N.D. +0.8 -0.2 -0.5 -0.1
stress 3-octanone 6.3476 mM N.D. +0.6 -0.3 -0.4 +0.0
stress 2-pentyl-furan 1.335528 mM N.D. +0.6 -0.4 -0.3 +0.1
stress 2-pentyl-furan 1.780666667 mM N.D. +0.6 -0.5 -0.1 +0.0
nitrogen source Glucuronamide (N) N.D. -0.6 +0.2 +0.3 +0.1
nitrogen source L-Citrulline (N) N.D. -0.3 +0.4 +0.1 +0.2
stress Paraquat dichloride 0.04 mg/ml N.D. +0.4 +0.3 -0.4 +0.2
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM N.D. +0.8 -0.4 +0.1 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. -0.2 +0.3 +0.4 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. -0.3 +0.1 +0.6 +0.3
lb LB N.D. -0.3 +0.4 +0.5 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.1 +0.7 +0.2 +0.2
motility inner cut, LB soft agar motility assay N.D. +0.6 +0.8 -0.2 -0.1
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