Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS04795 and AZOBR_RS04800 are separated by 41 nucleotidesAZOBR_RS04800 and AZOBR_RS04805 are separated by 18 nucleotidesAZOBR_RS04805 and AZOBR_RS04810 are separated by 60 nucleotidesAZOBR_RS04810 and AZOBR_RS04815 are separated by 181 nucleotides AZOBR_RS04795: AZOBR_RS04795 - acetoin:2 6-dichlorophenolindophenol oxidoreductase subunit alpha, at 1,008,394 to 1,009,377 _RS04795 AZOBR_RS04800: AZOBR_RS04800 - pyruvate dehydrogenase subunit beta, at 1,009,419 to 1,010,450 _RS04800 AZOBR_RS04805: AZOBR_RS04805 - dihydrolipoyllysine acetyltransferase, at 1,010,469 to 1,011,602 _RS04805 AZOBR_RS04810: AZOBR_RS04810 - shikimate 5-dehydrogenase, at 1,011,663 to 1,012,448 _RS04810 AZOBR_RS04815: AZOBR_RS04815 - radical SAM protein, at 1,012,630 to 1,013,547 _RS04815
Group Condition AZOBR_RS04795 AZOBR_RS04800 AZOBR_RS04805 AZOBR_RS04810 AZOBR_RS04815
stress 2-pentyl-furan 2.671 mM +0.0 -1.0 +0.3 N.D. -0.7
nitrogen source L-Isoleucine (N) -0.4 -0.1 +0.2 N.D. -0.8
stress 2-pentyl-furan 1.780666667 mM -0.1 -0.4 -0.3 N.D. -0.4
nitrogen source casaminos (N) -0.3 -0.2 -0.2 N.D. -0.3
carbon source D,L-Lactate (C) -0.2 -0.3 +0.0 N.D. -0.4
stress 3-octanone 4.2317 mM -0.3 -0.6 -0.1 N.D. +0.1
stress 3-octanone 6.3476 mM -0.6 -0.4 -0.1 N.D. +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.4 -0.5 +0.1 N.D. +0.0
nitrogen source L-Glutamic (N) +0.1 -0.2 -0.6 N.D. +0.1
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -0.5 +0.3 -0.4 N.D. -0.0
carbon source Glycerol (C) -0.4 -0.2 +0.3 N.D. -0.2
nitrogen source casaminos (N) +0.3 -0.3 -0.1 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.2 -0.2 N.D. +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.1 +0.2 N.D. -0.7
stress 2-pentyl-furan 1.780666667 mM -0.2 +0.3 -0.5 N.D. +0.1
nitrogen source L-Phenylalanine (N) +0.3 -0.2 +0.3 N.D. -0.5
nitrogen source L-Threonine (N) +0.2 +0.1 +0.1 N.D. -0.5
stress 2-pentyl-furan 1.335528 mM -0.5 +0.7 +0.0 N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.0 -0.4 N.D. +0.2
stress Sisomicin 0.01 mg/ml -0.6 +0.4 +0.5 N.D. -0.1
motility inner cut, LB soft agar motility assay +0.2 +0.5 -0.1 N.D. -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 -0.2 -0.3 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.3 +0.3 N.D. +0.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.3 +0.3 +0.1 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 +0.2 -0.2 N.D. +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +0.3 -0.2 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +0.5 -0.4 N.D. +0.3
stress Cobalt chloride 0.3 mM -0.0 +0.2 +0.2 N.D. +0.5
motility inner cut, LB soft agar motility assay +0.2 +0.0 +0.6 N.D. +0.1
stress Paraquat dichloride 0.04 mg/ml +0.3 +0.5 +0.2 N.D. +0.1
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