Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS04255 and AZOBR_RS04260 are separated by 131 nucleotidesAZOBR_RS04260 and AZOBR_RS04265 are separated by 346 nucleotidesAZOBR_RS04265 and AZOBR_RS04270 are separated by 16 nucleotidesAZOBR_RS04270 and AZOBR_RS04275 are separated by 53 nucleotides AZOBR_RS04255: AZOBR_RS04255 - hypothetical protein, at 897,750 to 898,715 _RS04255 AZOBR_RS04260: AZOBR_RS04260 - hypothetical protein, at 898,847 to 899,227 _RS04260 AZOBR_RS04265: AZOBR_RS04265 - 2-isopropylmalate synthase, at 899,574 to 901,178 _RS04265 AZOBR_RS04270: AZOBR_RS04270 - RNA methyltransferase, at 901,195 to 901,965 _RS04270 AZOBR_RS04275: AZOBR_RS04275 - response regulator, at 902,019 to 902,732 _RS04275
Group Condition AZOBR_RS04255 AZOBR_RS04260 AZOBR_RS04265 AZOBR_RS04270 AZOBR_RS04275
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. +0.1 -1.1 -4.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. -0.1 -1.8 -2.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 N.D. +0.0 -0.9 -2.9
carbon source D-Fructose (C) -0.8 N.D. -1.9 +0.1 -0.4
carbon source D-Fructose (C) -0.7 N.D. -2.0 +0.2 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -0.2 -0.6 -1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. -0.1 -1.8 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. +0.0 -1.5 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.1 N.D. -0.2 -0.6 -1.5
nitrogen source L-Lysine (N) -0.1 N.D. -1.6 -0.5 -0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.1 N.D. -0.8 -0.5 -1.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 N.D. -0.4 -0.8 -0.9
nitrogen source D-Alanine (N) -0.3 N.D. -0.5 -0.6 -0.9
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.1 N.D. -1.0 -0.3 -1.0
nitrogen source L-Glutamic (N) -0.6 N.D. -0.0 -1.2 -0.5
stress Sisomicin 0.01 mg/ml +0.3 N.D. -0.4 -0.6 -1.5
carbon source D,L-Lactate (C) -0.1 N.D. -0.4 -0.5 -1.1
nitrogen source L-Lysine (N) -0.1 N.D. -1.8 -0.1 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.0 N.D. -1.0 +0.1 -0.9
nitrogen source L-Isoleucine (N) -1.5 N.D. +0.1 +0.3 -0.6
carbon source pyruvate (C) +0.0 N.D. -0.6 -0.6 -0.5
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.0 N.D. -0.1 -1.9 +0.5
carbon source a-Ketoglutaric (C) -1.7 N.D. +0.2 +0.1 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 N.D. -1.2 +0.5 -0.5
carbon source a-Ketoglutaric (C) -1.6 N.D. +0.1 +0.9 -0.1
stress Cobalt chloride 0.3 mM +0.3 N.D. +0.3 -1.0 -0.2
carbon source Glycerol (C) -0.7 N.D. +0.5 -0.5 +0.2
motility inner cut, LB soft agar motility assay -0.2 N.D. -0.1 -0.9 +1.0
stress 2-pentyl-furan 2.671 mM +0.3 N.D. -0.1 -0.6 +0.8
motility outer cut, LB soft agar motility assay -0.4 N.D. +0.4 -0.2 +1.2
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