Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS04225 and AZOBR_RS04230 are separated by 99 nucleotidesAZOBR_RS04230 and AZOBR_RS04235 are separated by 402 nucleotidesAZOBR_RS04235 and AZOBR_RS04240 are separated by 75 nucleotidesAZOBR_RS04240 and AZOBR_RS04245 are separated by 4 nucleotides AZOBR_RS04225: AZOBR_RS04225 - isoprenoid biosynthesis protein, at 889,250 to 889,918 _RS04225 AZOBR_RS04230: AZOBR_RS04230 - indole-3-pyruvate decarboxylase, at 890,018 to 891,655 _RS04230 AZOBR_RS04235: AZOBR_RS04235 - glutamyl-tRNA synthetase, at 892,058 to 893,416 _RS04235 AZOBR_RS04240: AZOBR_RS04240 - cysteinyl-tRNA synthetase, at 893,492 to 894,865 _RS04240 AZOBR_RS04245: AZOBR_RS04245 - putative acyltransferase 3, at 894,870 to 896,066 _RS04245
Group Condition AZOBR_RS04225 AZOBR_RS04230 AZOBR_RS04235 AZOBR_RS04240 AZOBR_RS04245
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.7 N.D. N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.5 N.D. N.D. -0.0
motility outer cut, LB soft agar motility assay +0.0 -1.1 N.D. N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.2 N.D. N.D. -0.5
nitrogen source L-Phenylalanine (N) -0.4 -0.5 N.D. N.D. -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.6 N.D. N.D. -0.5
stress Aluminum chloride 5 mM -0.5 -0.3 N.D. N.D. -0.2
nitrogen source Cytosine (N) -0.3 -0.4 N.D. N.D. +0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 -0.5 N.D. N.D. +0.0
nitrogen source D-Alanine (N) -0.4 -0.5 N.D. N.D. +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.6 +0.1 N.D. N.D. -0.3
carbon source L-Malic (C) -0.3 -0.6 N.D. N.D. +0.2
nitrogen source L-Aspartic Acid (N) -0.5 -0.3 N.D. N.D. +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.5 -0.1 N.D. N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.4 N.D. N.D. -0.2
nitrogen source L-Leucine (N) +0.3 -0.3 N.D. N.D. -0.2
motility inner cut, LB soft agar motility assay +0.2 +0.2 N.D. N.D. -0.5
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.1 +0.4 N.D. N.D. -0.2
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -0.4 +0.3 N.D. N.D. +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.4 +0.2 N.D. N.D. +0.6
stress Cobalt chloride 0.3 mM +0.2 +0.5 N.D. N.D. -0.3
lb LB -0.3 +0.1 N.D. N.D. +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 +0.0 N.D. N.D. -0.1
stress D-Cycloserine 0.125 mg/ml +0.3 +0.5 N.D. N.D. -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.2 +0.1 N.D. N.D. +0.4
nitrogen source L-Isoleucine (N) +0.4 +0.2 N.D. N.D. +0.3
stress 2-pentyl-furan 1.335528 mM +0.6 +0.3 N.D. N.D. +0.0
motility inner cut, LB soft agar motility assay -0.6 +0.8 N.D. N.D. +0.6
stress Sisomicin 0.01 mg/ml +0.4 +0.2 N.D. N.D. +0.3
stress Cobalt chloride 0.2 mM +0.6 +0.3 N.D. N.D. +0.1
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