Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS04065 and AZOBR_RS04070 are separated by 95 nucleotidesAZOBR_RS04070 and AZOBR_RS04075 are separated by 117 nucleotidesAZOBR_RS04075 and AZOBR_RS04080 are separated by 195 nucleotidesAZOBR_RS04080 and AZOBR_RS04085 are separated by 108 nucleotides AZOBR_RS04065: AZOBR_RS04065 - ribosome-associated protein IOJAP, at 856,244 to 856,585 _RS04065 AZOBR_RS04070: AZOBR_RS04070 - nicotinic acid mononucleotide adenylyltransferase, at 856,681 to 857,298 _RS04070 AZOBR_RS04075: AZOBR_RS04075 - gamma-glutamyl phosphate reductase, at 857,416 to 858,696 _RS04075 AZOBR_RS04080: AZOBR_RS04080 - cytochrome C, at 858,892 to 859,758 _RS04080 AZOBR_RS04085: AZOBR_RS04085 - membrane protein, at 859,867 to 860,610 _RS04085
Group Condition AZOBR_RS04065 AZOBR_RS04070 AZOBR_RS04075 AZOBR_RS04080 AZOBR_RS04085
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.2 N.D. N.D. -0.0 -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 N.D. N.D. -0.8 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 N.D. N.D. -0.4 -0.2
stress Cobalt chloride 0.2 mM -0.8 N.D. N.D. -0.7 +0.1
stress Paraquat dichloride 0.02 mg/ml +0.2 N.D. N.D. +0.2 -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 N.D. N.D. -0.5 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. N.D. -0.6 -0.1
anaerobic; rich media LB; anaerobic -0.4 N.D. N.D. -0.2 -0.6
lb LB -1.0 N.D. N.D. -0.0 -0.1
carbon source L-Leucine (C) -0.1 N.D. N.D. -0.2 -0.8
stress Chlorite 1 mM -0.5 N.D. N.D. +0.2 -0.5
stress Cobalt chloride 0.3 mM -0.5 N.D. N.D. +0.4 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 N.D. N.D. -0.2 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. N.D. -1.1 +0.6
carbon source D-Gluconic Acid (C) -0.5 N.D. N.D. -0.6 +0.7
motility inner cut, LB soft agar motility assay -0.8 N.D. N.D. +0.1 +0.5
stress 2-pentyl-furan 1.780666667 mM -0.3 N.D. N.D. +0.5 -0.2
motility outer cut, LB soft agar motility assay -0.5 N.D. N.D. -0.1 +0.6
motility inner cut, LB soft agar motility assay -0.8 N.D. N.D. -0.1 +1.0
stress 3-octanone 6.3476 mM +0.6 N.D. N.D. -0.0 -0.5
nitrogen source casaminos (N) -0.4 N.D. N.D. +0.4 +0.3
nitrogen source Ammonium chloride (N) -0.3 N.D. N.D. +0.5 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.9 N.D. N.D. +0.6 -1.0
stress 2-Furfuraldehyde 0.2 vol% +0.4 N.D. N.D. +0.5 -0.3
stress Paraquat dichloride 0.04 mg/ml -0.3 N.D. N.D. +0.2 +0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.5 N.D. N.D. +0.1 +0.5
nitrogen source L-Serine (N) +0.3 N.D. N.D. +0.6 +0.2
nitrogen source L-Threonine (N) +0.8 N.D. N.D. +0.2 +0.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.9 N.D. N.D. +0.1 +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.8 N.D. N.D. +0.5 +0.9
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